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EP38462
Poster Title: N-glycoproteome from a cancer cell line and its non-tumorigenic cell line combining Fbs1-GYR N-glycopeptide enrichment and trapped-ionmobility- quadrupole-time-of-flight
Submitted on 09 Mar 2022
Author(s): Diego Assis1, Minyong Chen2, Shourjo Ghose1, Elizabeth Gordon1, Samuelson Jim2, Taron Chris2, Matthew Willetts1
Affiliations: 1. Bruker Daltonics, Billerica, MA, USA 2. New England Biolabs, Ipswich, MA, USA
Poster Views: 111
Submitted on 09 Mar 2022
Author(s): Diego Assis1, Minyong Chen2, Shourjo Ghose1, Elizabeth Gordon1, Samuelson Jim2, Taron Chris2, Matthew Willetts1
Affiliations: 1. Bruker Daltonics, Billerica, MA, USA 2. New England Biolabs, Ipswich, MA, USA
Poster Views: 111
Abstract: N-glycosylation is implicated in the development and progression of many cancer types and efficient methodologies are required for this type of study. Our group described in 2017 that Fbs1-GYR enrichment outperformed theestablished lectin enrichment method and offered a deeper analysis and greater coverage of the human serum N-glycoproteome. Fbs1 recognizes the core motif Man3GlcNAc2 preferring high mannose N-glycans. On the other hand, analysis of N-glycopeptides is still an analytical challenge for LC-MS/MS with respect to electrospray ionization, chromatographic separation and structural elucidation by collision induced dissociation experiments requiring improvements in the capabilities of mass spectrometry. In this work, we have combined Fbs1-GYR Nglycopeptide enrichment technology with parallel accumulation serial fragmentation (PASEF) on a trapped ion mobility spectrometry – quadrupole time-of-flight mass spectrometer to study the comprehensive glycopeptide profiles in HCT116 cancer cells and their non-tumorigenic DNMT1/3b double knockout cells (DKO1) Summary: In this work, we have combined Fbs1-GYR Nglycopeptide enrichment technology with parallel accumulation serial fragmentation (PASEF) on a trapped ion mobility spectrometry – quadrupole time-of-flight mass spectrometer to study the comprehensive glycopeptide profiles in HCT116 cancer cells and their non-tumorigenic DNMT1/3b double knockout cells (DKO1)References: Chenet.al.,2017,NatureCommunications - 10.1038/ncomms15487
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