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NEW INSULAR RED PROPOLIS FROM COLOMBIA: BOTANICAL ORIGIN, BIOLOGICAL  AND CHEMICAL MARKERS
EP29411
Poster Title: NEW INSULAR RED PROPOLIS FROM COLOMBIA: BOTANICAL ORIGIN, BIOLOGICAL AND CHEMICAL MARKERS
Submitted on 26 Nov 2018
Author(s): Salamanca Grosso, G.; Osorio Tangafarife, M.P.
Affiliations: Universidad del Tolima;
This poster was presented at 30th International Symposium on the Chemistry of Natural Products
Poster Views: 408
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Poster Information
Abstract: Prospection of the phytochemical components of EEP from Red Propolis from San Andres and its fractions detected the presence of phenolic compounds (Phenols flavonoids, flavones and flavonols). Total EERP 23.7 g/100 g. T. Phenols (Folina-Ciocalteu): 233.3 mg EGA/g and Flavonoids (Aluminiun tricloride): 50 mg E.Q/g Extract. IR-FT show particular absorption 3450, 1637 and 1633 also 1275 and 1048 cm-1. HPLC and HPTLC show 21 chemical compounds and ESI(-)-MS, and LC-ESI(-)-MS/MS of colombian red propolis, have the same chemical finger print and characteristic ion fragmentation patterm at m/z: 213, 255, 269, 271, 283, 313, 315, 339, 371, 373, 401, 429, 501, 502 y 503), for pinocembrine, biochanine A (283), formononetin (267), daidzein, They have also been identified in red propolis samples in the northeast of Brazil, Alagoas/Sergipe). In addition there were signs characteristics of molecular markers coincident with liquiritigenin (m/z: 119, 135, 255), vestitol, neovestitol, artellipin C (m/z: 200, 255, 299), quercetin (m/z: 151, 169, 179, 193, 211, 213, 229, 239, 257, 273, 283), guttiferone E, p-coumaric acid (m/z: 93, 119, 163), el 3-methoxy-4-hydroxy cinnamaldehyde (m/z: 105, 121, 133, 134, 149, 162, 177), el 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (m/z: 146, 160, 170, 174, 185, 211, 229), á 3-prenyl-4-hydroxy cinnamic acid (m/z: 119, 132, 176, 187, 231), crisin (m/z: 107, 119, 143, 145, 208 y 253), pinocembrin (m/z:107, 136, 145, 151, 164, 171, 187, 213, 231 y 255), 3,5-diprenyl-4-hydroxy cinnamic acid (m/z: 145, 200, 244, 255, 284 y 299) and dicaffeoylquinic acid (m/z: 135, 155, 161, 173, 179, 191, 353 y 515). Inspection of the 1H-NMR spectra allows one to obtain information regarding chemical heterogeneity of the sample as highlighted for the discrepant respect to resonance at the aliphatic and aromatic region. Qualitative analytical pattern by 1H-NMR for Colombian are different respect to Brazilian an Cuban red propolis samples.



Summary: The red propolis (PR), is a particular type that has proven an important biological activity under the metabolites according to their origin. It has been report as a special product from Cuba (2001), Venezuela (Andes Trujillo State, 2004), Brazil (Mangrovea area also in Paraiba, 2007 and 2008), México (Champoton, Campeche, 2010), China (Shandong, 2012) and Nigeria (Ogun, Delta, Rivers, Cross Rivers, 2017).References: A. Daugsch, C.S. Moraes, P. Fort, Y.K. Park Brazilian red propolis-chemical composition and botanical origin Evid Based Complement Altern Med, 5 (2008), pp. 435-441.

O. Cuesta-Rubio, A.L. Piccinelli, M.C. Fernandez, I.M. Hernández, A. Rosado, L. Rastrelli
Chemical characterization of Cuban propolis by HPLC-PDA, HPLC-MS, and NMR: the brown, red, and yellow Cuban varieties of propolis J Agric Food Chem, 55 (2007), pp. 7502-7509
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