Posters
« Back
Oligonucleotide Purification Strategies using a New High-Capacity Anion Exchange Resin
EP20160
Poster Title: Oligonucleotide Purification Strategies using a New High-Capacity Anion Exchange Resin
Submitted on 19 Dec 2013
Author(s): M. Deetz, J. Fisher, A. Gehris, J. Maikner and M. Kinzey
Affiliations: Advanced Biosciences
Poster Views: 838
View poster »


Poster Information
Abstract: With the advent of nucleic acid silencing technologies and the need for high purity diagnostic and therapeutic oligonucleotides, there is a need for high-capacity chromatographic supports that can deliver economic purification processes.

A new, 30 micron, mono-sized, polymeric resin has been recently developed that provides high resolution and high capacity for synthetic oligonucleotides. Physical properties of this new resin will be described, including particle size uniformity, ion exchange capacity, pressure stability, and chemical stability.

The purification of two synthetic 12mer DNA oligonucleotides (AAA CCT GAT GTG and GGT GGT GGT GGT) will be demonstrated, and will compare yields and purities to those from commercially available anion exchange resins.
Summary: A new, 30 micron, mono-sized, polymeric resin has been recently developed that provides high resolution and high capacity for synthetic oligonucleotides. Physical properties of this new resin will be described, including particle size uniformity, ion exchange capacity, pressure stability, and chemical stability. The purification of two synthetic 12mer DNA oligonucleotides will be demonstrated, and will compare yields and purities to those from commercially available anion exchange resins.Report abuse »
Creative Commons

Related Posters


Genetic Engineering in Male Sterility for Hybrid Variety Development
Abir Hasan Joy

VITVO: Mimicking In Vivo Complexity By The Innovative 3D Model
Olivia Candini1, Giulia Grisendi1, Elisabetta Manuela Foppiani1, Matteo Brogli1, Beatrice Aramini2, Valentina Masciale3, Carlotta Spano1, Tiziana Petrachi4, Elena Veronesi4, Pierfranco Conte5,6, Giorgio Mari1 & Massimo Dominici1,3

BAT Molecular Imaging with SPECT-CT, PET-CT, PET-MRI and Fluorescence-PET: A Systematic Review of the Literature Data
Tarik Z Belhocine, MD., Ph.D *, Albert A Driedger, MD., Ph.D **, Jean-Luc Urbain, MD., Ph.D ***

Multiplex miRNA Profiling for Biomarker Discovery and Verification Studies Using the FirePlex® Platform
M. Tackett, B. Heinrich, I. Diwan, G. Tejada, C. Rafferty, E. Atabakhsh, and D. Pregibon

Accelerating high-throughput screening with FirePlex®-HT:An automatable, multiplex immunoassay using FirePlex® Particle Technology
A. Perea, B. Heinrich, W. Austin, C. Rafferty, M. Camilleri, L. To, E. Atabakhsh, and D. Pregibon