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Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
EP30831
Poster Title: Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Submitted on 15 Oct 2019
Author(s): Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A
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This poster was presented at PEACe (Protein Expression in Animal Cells) Conference, 2019
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Abstract: Here, we present case studies on the optimization of ion channel and GPCR expression using the Expi293 and ExpiCHO Expression Systems. Additionally, we demonstrate the utility of a new range of Expi293 reagents, including engineered Expi293 cell lines (e.g. GNTI-, Inducible and Inducible/GNTI- Expi293 cell lines) to allow for regulated expression and/or glycosylation of membrane proteins. Together, these protein expression tools significantly enhance the ability of researchers to study membrane protein biology.Summary: Membrane proteins are a notoriously difficult class of proteins to express, purify and characterize. Expression levels of membrane proteins in mammalian expression systems, the preferred system to generate native-like posttranslational modifications, are often so low that yields are unsuitable for the intended downstream uses.Report abuse »
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