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EP30831
Poster Title: Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Submitted on 15 Oct 2019
Author(s): Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A
Affiliations:
This poster was presented at PEACe (Protein Expression in Animal Cells) Conference, 2019
Poster Views: 121
Submitted on 15 Oct 2019
Author(s): Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A
Affiliations:
This poster was presented at PEACe (Protein Expression in Animal Cells) Conference, 2019
Poster Views: 121
Abstract: Here, we present case studies on the optimization of ion channel and GPCR expression using the Expi293 and ExpiCHO Expression Systems. Additionally, we demonstrate the utility of a new range of Expi293 reagents, including engineered Expi293 cell lines (e.g. GNTI-, Inducible and Inducible/GNTI- Expi293 cell lines) to allow for regulated expression and/or glycosylation of membrane proteins. Together, these protein expression tools significantly enhance the ability of researchers to study membrane protein biology.Summary: Membrane proteins are a notoriously difficult class of proteins to express, purify and characterize. Expression levels of membrane proteins in mammalian expression systems, the preferred system to generate native-like posttranslational modifications, are often so low that yields are unsuitable for the intended downstream uses.
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