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Optimizing the hERG Assay on the PatchXpress 7000A System for Compounds that Demonstrate Non-specific Binding
EP20073
Poster Title: Optimizing the hERG Assay on the PatchXpress 7000A System for Compounds that Demonstrate Non-specific Binding
Submitted on 19 Dec 2013
Author(s): Iris Yang, Naibo Yang, Cathy Smith-Maxwell, Claire Quinn, Kirsty Macfarlane and David Yamane
Affiliations: Molecular Devices
Poster Views: 955
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Poster Information
Abstract: Non-specific binding of compound can affect the potency measurement from both conventional and automated patch-clamp assays. Because of this, pre-cautions need to be taken to ensure this is minimized or eliminated. Eight compounds, astemizole, pimozide, dofetilide, cisapride, terfenadine, flunarizine, quinidine and imipramine were used to evaluate the effect of different assay conditions to overcome the effects of non-specific binding in our hERG assay on the PatchXpress 7000A system.

To achieve the best IC50 values, in addition to optimizing instrument parameters to minimize non-specific binding, care had to be taken in all phases of compound preparation. Compound binding to plastic container need to be minimized. Buffers used for the assay also need to be optimized. In this poster, we present the assay conditions found that generated the best IC50 values on the PatchXpress 7000A as well as the assay results for these compounds.
Summary: Eight compounds, astemizole, pimozide, dofetilide, cisapride, terfenadine, flunarizine, quinidine and imipramine were used to evaluate the effect of different assay conditions to overcome the effects of non-specific binding in our hERG assay on the PatchXpress 7000A system.Report abuse »
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