We've updated our Privacy Policy to make it clearer how we use your personal data.
We use cookies to provide you with a better experience, read our Cookie Policy
EP29951
Poster Title: PEAKS™ Software for the processing of timsTOF Pro PASEF data: identification and label-free quantitation
Submitted on 11 Apr 2019
Author(s): Markus Lubeck(1), Heiner Koch(1), Scarlet Beck(1), Paul Savage(2), Oliver Raether(1), Pierre-Olivier Schmit(2) Paul Shan (3) and Gary Kruppa (4)
Affiliations: (1) Bruker Daltoniks GmbH, Bremen-Germany, (2) Bruker France SA, Wissembourg, France, (3) Bioinformatics Solutions, Waterloo, Canada, (4) Bruker Daltonics Inc, Billerica, USA
Poster Views: 628
Submitted on 11 Apr 2019
Author(s): Markus Lubeck(1), Heiner Koch(1), Scarlet Beck(1), Paul Savage(2), Oliver Raether(1), Pierre-Olivier Schmit(2) Paul Shan (3) and Gary Kruppa (4)
Affiliations: (1) Bruker Daltoniks GmbH, Bremen-Germany, (2) Bruker France SA, Wissembourg, France, (3) Bioinformatics Solutions, Waterloo, Canada, (4) Bruker Daltonics Inc, Billerica, USA
Poster Views: 628
Abstract: The performance of a timsTOFPro instrument with PASEF for shotgun proteomics (Bruker Daltonics) has been evaluated using tryptic digests of human cancer cell lysates (HeLa) (Pierce) and mouse heart cell digests. Chromatographic separations were performed on a nanoElute (Bruker) nano-UHPLC using a 25 cm, 75 μm ID Odyssey C18 nanocolumn with integrated emitter (IonOpticks, Australia) at 400 nl/min and column oven at 50 °C with direct loading. Data was analyzed using multiple interations of the PEAKS (www.bioinfor.com) and Perseus (Jürgen Cox, Max Planck Institute of Biochemistry) software. Results were normalized to <1% FDR on PSMs.
Summary: Here we describe how a modified version of the well-known PEAKS™ proteomics processing software suite can be used for the processing of PASEF datasets.
Summary: Here we describe how a modified version of the well-known PEAKS™ proteomics processing software suite can be used for the processing of PASEF datasets.
Ask the author a question about this poster.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Related Posters
Streamlining Biologics Development with the Expi Expression Systems
Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Kenneth Thompson, Maya Yovcheva, Sara Barnes, Mark Bundy, Melissa Cross, Katy Irvin, Joaquín Canay, Mary Reynolds, Natasha Lucki, Henry Chiou, Andrew Campbell, Jonathan Zmuda
ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda
Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A
2D FT0ICR MS/MS analysis of IgG1
Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1
High speed untargeted 4D-lipidomics LC-MS/MS workflows with Parallel Accumulation Serial Fragmentation (PASEF)
Ulrike Schweiger-Hufnagel1, Aiko Barsch1, Sven W. Meyer1