Posters
« Back
Peptidic and Non-Peptidic Ligand Activated GPCRs-Comparison of Assay Platforms
EP20439
Poster Title: Peptidic and Non-Peptidic Ligand Activated GPCRs-Comparison of Assay Platforms
Submitted on 19 Dec 2013
Author(s): Ellery JM, Kahn D, Hendrick A, Rapley H, Mitchell P, Barnes MJ
Affiliations: Paradigm Therapeutics Ltd
Poster Views: 1,289
View poster »


Poster Information
Abstract: A number of GPCRs activated by peptide ligands were assessed using different assay technologies.It was observed that the Ec50 values obtained using htrf assays were significantly higher than those obtained with other assay platforms. This difference was not observed with a non-peptide liganded GPCR. This highlights that with the GPCRs tested the use of htrf technology may not be the most appropriate assay format for peptide liganded GPCRs.Summary: A number of GPCRs activated by peptide ligands were assessed using different assay technologies. It was observed that the Ec50 values obtained using htrf assays were significantly higher than those obtained with other assay platforms. This difference was not observed with a non-peptide liganded GPCR. This highlights that with the GPCRs tested the use of htrf technology may not be the most appropriate assay format for peptide liganded GPCRs.Report abuse »
Creative Commons