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Periodontal Pockets Microbiota qPCR Analysis at Different Stages of Periodontitis
EP20635
Poster Title: Periodontal Pockets Microbiota qPCR Analysis at Different Stages of Periodontitis
Submitted on 20 Dec 2013
Author(s): Oksana Zorina and Denis Rebrikov
Affiliations: DNA-Technology
Poster Views: 1,358
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Poster Information
Abstract: Six major periodontal pathogens were quantified (with standardization to human gDNA) in periodontal pockets of periodontitis patients and healthy subjects by original qPCR assay. Relative amount of pathogens to total bacterial mass increased for P.gingivalis (100-fold), P.intermedia (10-fold) and T.forsythensis (10-fold).
Summary: Six major periodontal pathogens were quantified (with standardization to human gDNA) in periodontal pockets of periodontitis patients and healthy subjects by original qPCR assay. Relative amount of pathogens to total bacterial mass increased for P.gingivalis (100-fold), P.intermedia (10-fold) and T.forsythensis (10-fold).
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