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Picking the best CRISPR-Cas9 targets for functional gene knockout: a machine learning algorithm based on both specificity and functionality
EP23430
Poster Title: Picking the best CRISPR-Cas9 targets for functional gene knockout: a machine learning algorithm based on both specificity and functionality
Submitted on 24 Sep 2015
Author(s): Shawn McClelland, Emily M. Anderson, ┼Żaklina Strezoska, Elena Maksimova, Annaleen Vermeulen, Steve Lenger, Tyler Reed, and Anja van Brabant Smith Dharmacon, now part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US
Affiliations: GE Healthcare Dharmacon, Inc.
This poster was presented at Discovery On Target 2015
Poster Views: 4,599
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Poster Information
Abstract: Functional gene knockout is an important tool for understanding a gene's role in a system or for specifically manipulating a known system to achieve a desired outcome. Not all gene cleavage events result in functional knockout of the target protein. Here we share important advancements that have helped to achieve the goal of picking the best crRNA targets for functional gene knockout, and not just formation of indels (insertions or the deletions of bases in the DNA).
Summary: The CRISPR-Cas9 system has the potential to significantly advance basic and applied research. Report abuse »
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