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Platinum™ II Taq Hot-Start DNA Polymerase: PCR simplified with universal annealing
EP29308
Poster Title: Platinum™ II Taq Hot-Start DNA Polymerase: PCR simplified with universal annealing
Submitted on 17 Oct 2018
Author(s): Skaistė Valinskytė, Edita Elijošiūtė, Tomas Radzvilavičius, Miglė Mikutėnaitė, Jonas Belevičius, Aistė Serapinaitė, Rasa Sukackaitė
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Poster Information
Abstract: Platinum II Taq Hot-Start DNA Polymerase is designed for fast co-cycling of the PCR assays by two innovative technologies. First, Taq enzyme is enginereered by in vitro evolution for faster DNA synthesis and inhibitor resistance. Second, Platinum II PCR buffer contains isostabilizing molecules, which increase primer–template duplex stability during the annealing step. This enables optimal and specific binding of primers with different melting temperatures at universal annealing temperature (60ºC). As a result, the need to optimize the annealing temperature for each primer pair is eliminated. Instead, different assays can be run using the same primer annealing temperature and the same elongation time for amplicons of different lengths.Summary: When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step. References: Special thanks to J. Bagdanavičiūtė-Baliukevič, R. Leipuvienė, V. Žepnickaitė, E. Astromskas, S. Leisso, S. Siliūnas, E. Armalytė, A. Arlauskas, E. Jasaitė, M. Narmontas, A. Lagunavičius, R. Rimšelienė, R. Skirgaila, A. Mazūrienė, J. Niedritis, T. Kavaliūnienė, L. Paliukonienė, G. Mikšytė, J. Rimkienė, G. Sotvaraitė, K. Wiederholt, T. Zhu, and E. Šmergelienė.Report abuse »
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