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Platinum™ SuperFi™ DNA Polymerase for the highest success in PCR
EP29928
Poster Title: Platinum™ SuperFi™ DNA Polymerase for the highest success in PCR
Submitted on 08 Apr 2019
Author(s): Rasa Sukackaitė, Martyna Simutytė, Skaistė Valinskytė, Laurynas Vanagas, Karolis Matjošaitis, Renata Rimšelienė, Remigijus Skirgaila.
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Poster Views: 609
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Poster Information
Abstract: Platinum ™ SuperFi ™ DNA Polymerase is a new proofreading DNA polymerase, which combines superior fidelity with Platinum ™ hot-start technology.Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, template generation for sequencing and other applications requiring high accuracy. The product is based on new technologies providing top fidelity, specificity and yield: engineered DNA polymerase, Platinum hot-start technology and GC enhancer. The Platinum SuperFi DNA Polymerase was engineered by mutagenesis and fusion of a DNA-binding domain for extremely low error rate and high processivity. High processivity enables fast cycling protocols, amplification of long targets and increased resistance to inhibitors.Summary: Platinum SuperFi DNA Polymerase shows leading performance for the highest success in PCR.
• Fidelity: minimal sequence error rate
• Specificity: minimum non-targeted bands even after incubation of PCR reaction at room temperature
• Yield: high yield even from low template amounts
• Difficult targets: long, GC rich, in presence of inhibitors
• Convenience: room temperature set up, 2X master mix, fast cycling, direct gel loading.
References: Vilija Drėgūnienė, Greta Daujotaitė, Stephanie Leisso, Eimantas Astromskas, Milda Kaniušaitė, Jurgita Rubekina, Arūnas Lagunavičius, Dovilė Cicėnaitė, Šarūnas Streckis, Vilius Bešėnas, Agnė Mazūrienė, Tatjana Kavaliūnienė, Deividas Jasiulevičius, Nijolė Razmienė, Lina Šmigelskienė, Birutė Kaziliūnienė, Laura Paliukonienė, Dovilė Rekertienė, Gražina Mikšytė, Agnė Bujanauskaitė, Bernadette Perfect, Eglė Balčiūnaitė, Violeta Popendikytė, Gediminas AlzbutasReport abuse »
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