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Polydimetylsiloxane (PDMS) Surface Modifications for  Enhancing Lipopolysaccharide Stimulation in Cell Studies
EP25662
Poster Title: Polydimetylsiloxane (PDMS) Surface Modifications for Enhancing Lipopolysaccharide Stimulation in Cell Studies
Submitted on 01 Apr 2017
Author(s): Olja Simoska, P. Rogelio Escamilla, Janine Elliott, Eric V. Anslyn and Jason B. Shear
Affiliations: The University of Texas at Austin, Department of Chemistry
This poster was presented at 03/07/2017
Poster Views: 1,837
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Poster Information
Abstract: Polydimethylsiloxane (PDMS) is often used as a biocompatible polymer to create devices for cell culture experiments. Its hydrophobic surface, however, results in substantial non-specific adsorption of a range of hydrophobic biological molecules, particularly in relatively high surface area-to-volume PDMS-based devices. We have a specific interest in characterizing stimulation of macrophage-derived cells by lipopolysaccharide (LPS). a hydrophobic signal that promotes macrophage secretion of nitric oxide (NO), which functions as a key a biogenic control point. Thus, strategies for minimizing adsorption of LPS to PDMS surfaces in miniaturized chambers are fundamental to interpreting macrophage stimulation using electrochemical detection of NO. To address this requirement, we have functionalized PDMS surfaces by treatment with three different silane-based groups to enhance surface hydrophilicity. Here, we present the characterization of these modifications using XPS and other analytical methods. In addition, we performed cell studies with RAW 264.7 macrophage-derived cells using the three different fictionalized PDMS surfaces. The modifications introduced to the PDMS surface show enhancement in LPS stimulation.Summary: We have an interest in characterizing stimulation of macrophage-derived cells by lipopolysaccharide (LPS), a hydrophobic signal that promotes secretion of nitric oxide (NO). Strategies for minimizing adsorption of LPS to PDMS surfaces are fundamental to interpreting macrophage stimulation using electrochemical detection of NO. We have functionalized PDMS surfaces with three different silane groups to enhance surface hydrophilicity. The modifications show enhancement in LPS stimulation.Report abuse »
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