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Potential therapeutic effects of induced pluripotent stem cells on inducedsalivary gland cancer in experimental rats
EP29735
Poster Title: Potential therapeutic effects of induced pluripotent stem cells on induced salivary gland cancer in experimental rats
Submitted on 20 Feb 2019
Author(s): Yasmine Alaa El-Din1, Dina Sabry2, Amal Hassan Abdelrahman3, and Safa Fathy3
Affiliations: Oral Pathology Department1, October 6 University, Faculty of Dentistry- Giza, Egypt; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt2; Oral & Maxillofacial Pathology, Faculty of Dentistry, Cairo University, Cairo, Egypt3
This poster was presented at Faculty Of Dentistry- Cairo University
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Poster Information
Abstract: ABSTRACT
Salivary gland neoplasms exhibit complex histopathology in a variety of tumor types and treatment options depend largely on the stage of the cancer. Induced pluripotent stem cells (iPS) have been investigated for treating induced salivary gland cancer and for restoring salivary gland function. For our study in the vitro part, we re-programmed human skin fibroblasts to form iPS cells using a plasmid containing Oct4, Sox2, L-MYC and LIN28. In the vivo part, we used 30 white male albino rats divided into the following groups of 10: group 1 (control): rats were injected with phosphate buffered saline (PBS), group 2 induced squamous cell carcinoma (SCC): rat submandibular glands were injected with squamous carcinoma cells (SCC), group 3 (induced SCC/iPS): SCC rats treated with 5 × 106 iPS cells. Submandibular glands from rats of all groups were examined histologically and real time PCR was performed for amylase, and COX I and COX II gene expression. We confirmed that submandibular gland specimens included tumor tissue before starting treatment with iPS. iPS treated cases exhibited regeneration of salivary glands, although minor degenerative and vascularization changes remained. The acinar cells regained their proper organization, but continued to exhibit abnormal activity including hyperchromatism. iPS cells may be useful for treating salivary gland carcinomas.
Summary: This experiment was performed to study the potential therapeutic effects of induced pluripotent stem cells on induced salivary gland cancer
in experimental rats.
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h
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