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Medical Student: Predictive Biomarkers for the Clinical Evaluation of Bladder Cancer
EP30802
Poster Title: Medical Student: Predictive Biomarkers for the Clinical Evaluation of Bladder Cancer
Submitted on 15 Oct 2019
Author(s): Isha Dabke; Richard Pearce;, Natasha Venugopal; Georgios Kallifatidis; Bal Lokeshwar
Affiliations: Medical College of Georgia at Augusta University
Poster Views: 103
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Poster Information
Abstract: Bladder Cancer ranks second in mortality and morbidity among the genitourinary cancers. Currently, its diagnosis involves cystoscopy and biopsy, which can take weeks to yield results. Even imaging studies such as MRI and CT aren't sensitive enough to detect bladder tumors. Morphologically, bladder cancer can be divided into two subtypes: luminal and basal, which express distinct stem cell markers and have differing sensitivities to therapy. Although diagnosis based on molecular signatures has the potential to be effective, a verification of their expression in strictly compartmentalized epithelial subtypes is not presently available. The implications of this project are the formation of a bedside tool-kit based on prognostic biomarkers that can be used following biopsy to evaluate cancer subtype and provide a preliminary diagnosis.

We analyzed genomic expression profiles in five bladder cancer cell lines, ranging from grade I to grade IV cancers. Based on existing literature, these cell lines were classified into either the luminal or basal subtype. Importantly, basal bladder cancers are more aggressive and lead to shorter survival. Through RT-PCR and Western Blot studies, genomic expression levels of eleven biomarkers were analyzed in the cells.

Less aggressive/luminal cancers showed higher PCR/blot expression profiles for luminal biomarkers, and expressed GATA-3 100% of the time. The more invasive/basal cell lines expressed CD44S 100% of the time. Thus, the expression profiles of only two biomarkers (GATA-3 and CD44S) can be used to efficiently identify the molecular subtype of the patient's cancer to gauge a first-look prognosis at the bed-side before obtaining pathology results.
Summary: Cancer of the urinary bladder (BC) ranks second in mortality and morbidity among the genitourinary cancers causing over 16,000 deaths annually. It is the most expensive cancer to treat from diagnosis to death, due in part to its intrinsic molecular heterogeneity that makes prognosis difficult, requirement of invasive procedures such as cystoscopy, and a high incidence of recurrence. References: HIV and Older Adults Understanding HIV/AIDS. (2019, April 08). Retrieved from
https://aidsinfo.nih.gov/understanding-hiv-aids/fact-sheets/25/80/hiv-and-older-adults

HIV/AIDS. (2018, September 18). Retrieved from
https://www.cdc.gov/hiv/group/age/olderamericans/index.html

Ports, K. A., Barnack-Tavlaris, J. L., Syme, M. L., Perera, R. A., & Lafata, J. E. (2014). Sexual Health
Discussions with Older Adult Patients During Periodic Health Exams. The Journal of Sexual
Medicine,11(4), 901-908. doi:10.1111/jsm.12448

2017 Sexually Transmitted Diseases Surveillance. (n.d.). Retrieved from
https://www.cdc.gov/std/stats17/default.htm
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