Posters
« Back
Primary Human Hepatocytes 3D in vitro Culture Model for Studying Hepatic Function
EP30746
Poster Title: Primary Human Hepatocytes 3D in vitro Culture Model for Studying Hepatic Function
Submitted on 28 Sep 2019
Author(s): Sujoy Lahiri, Julia Tritapoe, Kate Comstock, Michael F. Millett, Mark Kennedy, Theresa V. Nguyen, Deborah K. Tieberg, David T. Kuninger
Affiliations: Thermo Fisher Scientific
This poster was presented at ISSX 2019
Poster Views: 274
View poster »


Poster Information
Abstract: Primary Human Hepatocyte (PHH) culture provides the closest in vitro model to human liver that can produce a metabolic profile of a given drug very similar to that found in vivo. Hence, PHH culture is the gold standard for studying the in vitro hepatic biology, liver function, and drug induced hepatotoxicity. The conventional way of culturing PHH in 2-dimension (2D) has major pitfalls. The PHH rapidly de-differentiate and lose the hepatic specific functions in a week. Therefore, there is a need for more robust in vitro models that reflect in vivo liver biology more accurately and maintains the liver functions for a longer time. 3-dimensional (3D) hepatic in vitro models have gained a lot of attention for their ability to recapitulate the hepatic function and greater longevity.

Recently we have developed an easy-to-assemble user-friendly in vitro Primary Human Hepatocyte (PHH) 3D-spheroid model. The 3D-hepatic spheroids are viable for at least 4 weeks in culture and remain phenotypically stable, retaining the hepatocyte-specific functions.
Summary: Primary Human Hepatocyte (PHH) culture provides the closest in vitro model to human liver that can produce a metabolic profile of a given drug very similar to that found in vivo. Recently we have developed an easy-to-assemble user-friendly in vitro Primary Human Hepatocyte (PHH) 3D-spheroid modelReferences: 1. Thermo Fisher Scientific. User Guide: Cryopreserved 3D-Spheroid Qualified Human Hepatocytes. Pub. No. MAN0018280.

2. Thermo Fisher Scientific. Thawing and Plating Cryopreserved Hepatocytes. Protocol available at: https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/thawing-and-plating-hepatocytes-protocol.html
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Streamlining Biologics Development with the Expi Expression Systems
Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Kenneth Thompson, Maya Yovcheva, Sara Barnes, Mark Bundy, Melissa Cross, Katy Irvin, Joaquín Canay, Mary Reynolds, Natasha Lucki, Henry Chiou, Andrew Campbell, Jonathan Zmuda

ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda

Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A

FINGERPRINT 1H- NMR BASED ON THE CHEMICAL PROFILING OF COLOMBIAN RED PROPOLIS
Guillermo Salamanca Grosso; Monica Patricia Osorio Tangarife; David Garcia Mendez; Julien Wist

Genetic Engineering in Male Sterility for Hybrid Variety Development
Abir Hasan Joy