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Pyroptosis Detection via Multiplexing Cell Death and Bioluminescent Caspase-1 Activity Assessments
EP25810
Poster Title: Pyroptosis Detection via Multiplexing Cell Death and Bioluminescent Caspase-1 Activity Assessments
Submitted on 25 May 2017
Author(s): Martha O’Brien1, Mike Scurria2, Tim Ugo2, Laurent Bernad2, James Cali1, and Dan Lazar1
Affiliations: 1 Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711; 2 Promega Biosciences LLC, 277 Granada Dr, San Luis Obispo, CA 93401
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Abstract: Pyroptosis is a unique immunogenic form of cell death dependent on inflammasome activation, provoked when pattern recognition receptors (PRRs) sense microbial infection or cellular stress. Caspase-1, an essential component of inflammasomes, mediates this innate immune response by processing cytokines and eliciting cell death. In addition to caspase-1, other inflammatory caspases (caspase-11 in mice and caspases-4, 5 in humans) can cleave gasdermin D and elicit pyroptosis in response to some intracellular microbial infections. However, caspase-1 is also activated under these circumstances, therefore caspase-1 activation coincident with cell death is a reliable indicator of pyroptosis. Understanding when PRR sensing leads to cell death and when it does not is essential to understanding the complexities of the innate immune response. We have developed a convenient method to monitor pyroptosis by multiplexing in the same assay well our homogeneous, bioluminescent caspase-1 activity assay with a membrane-impermeant, stable DNA dye for the detection of cell death. In contrast to endpoint assays for cell death such as lactate dehydrogenase (LDH) assays, the DNA dye can be used in real-time. Cell death can be monitored over time and when first detected, the caspase-1 assay can be performed confirming pyroptosis. Alternatively, culture medium can be removed for testing for caspase-1 release, leaving the cells available for measuring cell death by a variety of means. We show examples of pyroptosis in human THP-1 monocytes and mouse J774A.1 macrophages. Summary: Developed a convenient method to confirm pyroptosis

Inflammasome activation triggers rapid pyroptosis

Preventing pyroptosis may shift form of cell death
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