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Quality Analysis of Circulating Cell-Free DNA and RNA
Poster Title: Quality Analysis of Circulating Cell-Free DNA and RNA
Submitted on 16 Dec 2020
Author(s): Chava Pocernich and Steve Siembieda
Affiliations: Agilent Technologies
This poster was presented at Next Generation DX Summit Washington DC
Poster Views: 491
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Poster Information
Abstract: Liquid biopsy is gaining prevalence as a non-invasive alternative approach for screening of health-related issues. Samples for liquid biopsy can come from plasma, serum, urine, saliva, or cerebrospinal fluid, where small fragments of DNA and RNA have been released from cells. The most prevalent bio-markers isolated from liquid biopsy include circulating tumor cells, circulating tumor DNA, cell-free DNA (cfDNA), circulating endothelial cells, and exosomes containing RNA. Recent studies have shown that cfDNA fragmentation provides information about the cause and tissue of origin through the fragmentation pattern and sequence. Quality analysis ensures successful sequencing of precious samples. Investigation of sizing, concentration, and distribution patterns from circulating DNA and RNA from plasma and serum was performed with the Agilent Femto Pulse system. An extended nucleosome pattern was observed from cfDNA isolated from human serum. Additionally, quality analysis provided reliable sizing and concentration, while providing a visual assessment of the sample.
For Research Use Only. Not for use in diagnostic procedures.
Summary: Quality control analysis of cfDNA and cfRNA on the Agilent Femto Pulse SystemReport abuse »
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