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Quantitative Live-Cell Analysis Using Automated Long-Term Imaging
EP26132
Poster Title: Quantitative Live-Cell Analysis Using Automated Long-Term Imaging
Submitted on 11 Jul 2017
Author(s): Joe Clayton and Peter Banks
Affiliations: BioTek Instruments, Inc. Winooski, VT USA
Poster Views: 883
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Poster Information
Abstract: Characterizing cell proliferation is a crucial aspect of biological research and therapeutic drug development. Most current cell proliferation assays rely on indirect biochemical metrics that are limited by artifacts or imaging-based endpoint measures.

Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5. This fully automated method enables quantitative and phenotypic long-term analysis of cell growth using non-invasive measures of confluence or direct cell count. To demonstrate the abilities of this system to conduct robust and reproducible kinetic proliferation assays, NIH3T3, HeLa, and HCT116 cell growth was followed for five days. All three cell types exhibited robust logarithmic growth up to full confluence with doubling times consistent with literature values. Additionally, to demonstrate the ability of this system to screen pharmacological agents, cell proliferation profiles for cells cultured with eight concentrations of two literature-standard inhibitory compounds were generated. Calculated IC50 values were used to measure drug response for each compound and cell type.
Summary: Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5.References: Coupling the fully automated cell-handling abilities of the BioSpa 8 with the imaging and image analysis capabilities of the Cytation 5 provides a simple and powerful system to conduct long-term proliferation assays. This method enables accurate, label-free or fluorescence label-based cell growth measurements that can be readily scaled-up to meet the needs of high-throughput applications. Report abuse »
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