Posters
« Back
Rapid electrochemical impedance spectroscopy for protein detection in Lab-on-a-Chip devices
EP23905
Rapid electrochemical impedance spectroscopy for protein detection in Lab-on-a-Chip devices
Submitted on 21 Mar 2016

T. Pardy(a); N. Sleptsuk(a); M. Min(a); J. Ojarand(a); T. Lakspere(a,b); K. Palm(b)
(a) Thomas Johann Seebeck Department of Electronics, Tallinn University of Technology, Tallinn, Estonia; (b) Protobios OÜ, Tallinn, Estonia
This poster was presented at Lab-on-a-Chip & Microfluidics 2016
Poster Views: 978
View poster »
Poster Abstract
Lab-on-a-Chip devices form an ever-growing segment of the IVD market, and there is a pronounced need for reliable, rapid detection methods for various biomarkers, especially label-free methods. Electrochemical impedance spectroscopy (EIS) in liquids means the determination of passive electrical properties of ingredients in a continuous or segmented flow of fluids. More specifically, it means label-free discovery, counting and characterization of particles, mostly concentrations of various ionized molecules in chemical solutions or biological particles in fluids. We present results in rapid solution impedance spectroscopy to detect protein interactions (antibody-antigen) in human serum. The experimental setup was based on screen-printed electrodes (Dropsens DRP-C220AT) and cuvettes (Brandtech 7592 00), and the serum was buffered in PBS. Two different serum-antibody mixtures were created and impedance spectra recorded over 15 minutes to determine whether the system was capable of discerning solution compositions. Significant shifts in impedance magnitude and phase were detected in the 10 Hz-100 kHz range, with a clear difference between peaks for both solutions. Although in the described experiment, the solution was static, this setup could be adapted to be part of a flow cell.

[1] J. Jiang, X. Wang, R. Chao, Y. Ren, C. Hu, Z. Xu, and G. L. Liu, “Smartphone based portable bacteria preconcentrating microfluidic sensor and impedance sensing system,” Sensors and Actuators B: Chemical, vol. 193, pp. 653–659, Mar. 2014.
[2] X. Luo and J. J. Davis, “Electrical biosensors and the label free detection of protein disease biomarkers,” Chemical Society Reviews, vol. 42, no. 13, p. 5944, 2013.
[3] A. Bogomolova*, E. Komarova, K. Reber, T. Gerasimov, O. Yavuz, S. Bhatt and M. Aldissi, Challenges of Electrochemical Impedance Spectroscopy in Protein Biosensing, Anal. Chem., 2009, 81 (10), pp 3944–3949.
[4] Jonathan S. Daniels and Nader Pourmand, Label-Free Impedance Biosensors: Opportunities and Challenges. Electroanalysis. 2007 May 16; 19(12): 1239–1257.
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Screening of ALDH2 Interacting Proteins by Co-Immunoprecipitation and Mass Spectrometry
Irene John

Applying QbD in Process and Impurity Control Strategy Development
Andrew Anderson, Graham A. McGibbon, Sanjivanjit K. Bhal, and Joe DiMartino

ELECTROWETTING CHIP ON PAPER BASED SUBSTRATE
Nurul Amziah Md Yunus and Noor Faezah Ismail

Generating Unbiased Structural Alternatives for Automated Structure Verification
Sergey Golotvin, Rostislav Pol, Mikhail Elyashberg, Dimitris Argyropoulos and Karim Kassam

A Review Of Computer Assisted Structure Elucidation (CASE) Methodology
David C. Adams, Dimitris Argyropoulos