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Rapid Identification of Microorganisms by Touch Spray and Paper Spray Ambient Ionization
Poster Title: Rapid Identification of Microorganisms by Touch Spray and Paper Spray Ambient Ionization
Submitted on 26 Feb 2014
Author(s): Ahmed M. Hamid, Alan K. Jarmusch, Valentina Pirro and R. Graham Cooks
Affiliations: Purdue University
This poster was presented at Pittcon 2014
Poster Views: 1,900
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Poster Information
Abstract: Rapid and accurate identification of bacterial and yeast isolates is needed to advance public health. Touch spray is a novel ambient ionization method developed. It is performed by using any metal probe capable of transferring material from the object of interest to the surface of the probe for analysis. In this particular application, the bacterial colonies are directly sampled from culture dishes. Touch spray ionization can also be used to detect suspended micro-organisms in various solvents, such as methanol, water, dimethyl formamide and acetonitrile. Detected lipid profiles are characteristic for various bacteria genera. Touch spray has been utilized efficiently to distinguish among gram-negative and gram-positive bacteria in both of positive mode and negative modes. As an example of species differentiation, Staphylococcus aureus, Staphylococcus hominis, and Staphylococcus epidermidis are readily distinguished through their lipid patterns utilizing multivariant statistical analyses. In a parallel study, individual bacterial colonies are transferred to a filter paper cut into a sharp point and analyzed using paper spray-MS. Comparison of the mass-spectral data obtained from paper spray and touch spray ionization techniques show high degree of similarity and reproducibility.Summary: The rapid and accurate identification of microorganisms is necessary in order to improve public health. Molecular-based identification allows for rapid patient treatment and provides more accurate diagnoses, two crucial aspects for successful treatment, yet a great challenge. In this work PS-MS, an existing ambient method, and TS-MS, a newly developed technique, are being utilized in the rapid discrimination of microorganisms (<2 min).References: (1) Umeda, A.; Ueki, Y.; Amako, K. Journal of Bacteriology 1987, 169, 2482-2487.
(2) Havlicek, V.; Lemr, K.; Schug, K. A. Analytical Chemistry 2013, 85, 790-797.
(3) Rath, C.; Yang, J.; Alexandrov, T.; Dorrestein, P. J. Am. Soc. Mass Spectrom. 2013, 24, 1167-1176.
(4) Tang, Y. W.; Ellis, N. M.; Hopkins, M. K.; Smith, D. H.; Dodge, D. E.; Persing, D. H. Journal of Clinical Microbiology 1998, 36, 3674-3679.
(5) Espy, R. D.; Muliadi, A. R.; Ouyang, Z.; Cooks, R. G. International Jou
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