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Real-time search of Ubiquitin diGLY modified peptides and PaSER acquisition control on the timsTOF PRO
Poster Title: Real-time search of Ubiquitin diGLY modified peptides and PaSER acquisition control on the timsTOF PRO
Submitted on 09 Mar 2021
Author(s): Christopher Adams1; Robin Park1; Nagarjuna Nagaraj4; Sven Brehmer4; Matt Willetts3; Elizabeth Gordon3; Barry M. Zee2; Hayley Peckham2; Kimberly Lee2; Tharan Srikumar3 1Bruker,San Jose,CA;2Cell Signaling Technologies, Danvers, MA;3Bruker, Billerica, MA; 4Bruker, Bremen, Germany
This poster was presented at US HUPO 2021
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Poster Information
Abstract: Recently we introduced PaSER (PArallel Search Engine in Real-time) which performs ad atabase search in parallel with the 120Hz sequencing speed of thet imsTOFPro mass spectrometer. The PaSER box sits adjacent to any timsTOFPro or fleX instrument. Biological processes are influenced by the PTM state of proteins, one such PTM (ubiquitination) drives proten ubiquitinatedin catabolism. Phosphorylation is a critical signaling molecule acting at the time scale of seconds. Here we show PaSER has the computational power to real time assign peptides by identification of the remnant GlyGly tag on lysine residues. The search engine is fast enough to perform variable modification searches (Ox-M, Deam-NQ, Ubq-K) undersemi-tryptic search conditions allowing for 3 miscleavages and multiple modifications per peptide. Commonly, these search parameters come at a high computational time and costs.Summary: Ubiquitin modified peptides are observed in real time, Real-time search for even the most arduous PTM search parameters are capable using PaSER technology.References: Report abuse »
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