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Real-time search of Ubiquitin diGLY modified peptides and PaSER acquisition control on the timsTOF PRO
Poster Title: Real-time search of Ubiquitin diGLY modified peptides and PaSER acquisition control on the timsTOF PRO
Submitted on 05 Oct 2021
Author(s): Christopher Adams1; Robin Park1; Nagarjuna Nagaraj4; Sven Brehmer4; Matt Willetts3; Elizabeth Gordon3; Barry M. Zee2; Hayley Peckham2; Kimberly Lee2; Tharan Srikumar3
Affiliations: 1Bruker,San Jose,CA;2Cell Signaling Technologies, Danvers, MA;3Bruker, Billerica, MA; 4Bruker, Bremen, Germany
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Poster Information
Abstract: Recently we introduced PaSER (PArallel Search Engine in Real-time) which performs a database search in parallel withthe120Hz sequencing speed of the timsTOF Promass spectrometer. The PaSERbox sits adjacent to any timsTOF Pro or fleX instrument. Biological processes are influenced by the PTM state of proteins, one such PTM (ubiquitination) drives protein catabolism. Phosphorylation is a critical signaling molecule acting at the time scale of seconds. Here we show PaSER has the computational power to real time assign ubiquitinated peptides by identification of the remnant GlyGly tag on lysine residues. The search engine is fast enough to perform variable modification searches (Ox-M, Deam-NQ, Ubq-K) under semi-tryptic search conditions allowing for 3 miscleavages and multiple modifications per peptide. Commonly, these search parameters come at a high computational time and costs.Summary: •Ubiquitin modified peptides are observed in real time
•Real-time search for even the most arduous PTM search parameters are capable using PaSER technology.
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