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riboPOOL: Affordable Custom/Ribosomal RNA Depletion Against Any Species for RNA-Seq
Poster Title: riboPOOL: Affordable Custom/Ribosomal RNA Depletion Against Any Species for RNA-Seq
Submitted on 21 Mar 2019
Author(s): Catherine Goh1, Andrew Walsh1, Michaela Beitzinger1, Jonas Bertram1, Kristina Döring3, Sascha Dietrich3, Iana Kim4, Stefan Kotschote2, Claus Kuhn4, Konrad Förstner3, Michael Bonin2, Stefan Hannus1, Michael Hannus1
Affiliations: 1, siTOOLs Biotech GmbH; 2, IMGM Laboratories GmbH; 3, Core Unit Systems Medicine, University of Würzburg; 4, University of Bayreuth
This poster was presented at 9th Gene Quantification Event qPCR dPCR & NGS 2019
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Poster Information
Abstract: With the rising use of RNA Sequencing (RNA-Seq) with Next-Generation Sequencing (NGS), there is a growing demand for reagents that
increase output and reliability of sequencing data. Ribosomal RNAs (rRNAs) occupy more than half of total RNA sequencing reads. For
sensitive detection of scientifically relevant RNAs, rRNAs are removed either by physical depletion methods using rRNA-specific probes
or by isolating relevant coding RNAs through poly-A-tail affinity enrichment. Current commercial rRNA removal kits are costly and limited
to well-studied species. riboPOOLs developed by siTOOLs Biotech in partnership with IMGM Laboratories and scientists from University
of Würzburg and Bayreuth, present an affordable and flexible solution that gives scientists the freedom to deplete rRNAs or other
custom abundant RNAs from any species. Composed of high complexity pools of optimally-designed biotinylated DNA probes,
riboPOOLs specifically associate with cytoplasmic and mitochondrial rRNAs, enabling their removal with magnetic streptavidin-coated
beads. Human and mouse riboPOOLs were demonstrated to outperform current commercial rRNA depletion kits (Ribo-Zero, Illumina) in
RNA-Seq experiments, depleting ~10% more rRNA while leaving other RNA intact. riboPOOLs have been made against a diverse array of
organisms including Planarian, Silkworm, Drosophila, Arabidopsis and Bacteria. Furthermore, riboPOOLs can be tailored to deplete
abundant tissue-specific mRNAs, such as globin in red blood cells, making it applicable for NGS-based clinical diagnostics.
Summary: The design and production of rRNA depletion probes (riboPOOL) by siTOOLs Biotech was carried out and performance compared against current commercial solution (Ribo-Zero by Illumina) for human and mouse total RNA samples.Report abuse »
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