Posters
« Back
RNA Binding Protein Immunoprecipitation (RIP)
EP39318
Poster Title: RNA Binding Protein Immunoprecipitation (RIP)
Submitted on 27 Sep 2022
Author(s): Creative BioMart
Affiliations: Creative BioMart
This poster was presented at https://www.creativebiomart.net/resource/principle-protocol-rna-binding-protein-immunoprecipitation-rip-380.htm
Poster Views: 136
View poster »


Poster Information
Abstract: RNA binding protein immunoprecipitation (RIP) assay utilizes antibodies against the target protein to precipitate the corresponding RNA-protein complexes. The RNA bound to the complex can be validated by q-PCR or sequencing analysis after isolation and purification. RIP is a technique to study the combination of RNA and protein in cells. It is a powerful tool to understand the dynamic process of post-transcriptional regulation of the network, which can help us discover the regulatory targets of miRNAs.
Summary: The discovery of non-coding RNA makes RNA become the focus of life science research once again. Because RNA is an unstable biological macromolecule, most RNAs need to be bound to a specific RNA-binding protein to form an RNA / protein complex in order to be stable in cells. Furthermore, dynamic association between RNA and RNA-binding proteins throughout and accompanies the entire life cycle of RNA transcriptional synthesis, processing and modification, intracellular trafficking and localization,References: https://www.creativebiomart.net/resource/principle-protocol-rna-binding-protein-immunoprecipitation-rip-380.htmReport abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons