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RNA Binding Protein Immunoprecipitation (RIP)
Poster Title: RNA Binding Protein Immunoprecipitation (RIP)
Submitted on 27 Sep 2022
Author(s): Creative BioMart
Affiliations: Creative BioMart
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Abstract: RNA binding protein immunoprecipitation (RIP) assay utilizes antibodies against the target protein to precipitate the corresponding RNA-protein complexes. The RNA bound to the complex can be validated by q-PCR or sequencing analysis after isolation and purification. RIP is a technique to study the combination of RNA and protein in cells. It is a powerful tool to understand the dynamic process of post-transcriptional regulation of the network, which can help us discover the regulatory targets of miRNAs.
Summary: The discovery of non-coding RNA makes RNA become the focus of life science research once again. Because RNA is an unstable biological macromolecule, most RNAs need to be bound to a specific RNA-binding protein to form an RNA / protein complex in order to be stable in cells. Furthermore, dynamic association between RNA and RNA-binding proteins throughout and accompanies the entire life cycle of RNA transcriptional synthesis, processing and modification, intracellular trafficking and localization,References: abuse »
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