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Robust Glucose and 1,5-Anhydroglucitol Uptake Assays Using Novel Fluorescent Conjugates
EP27364
Poster Title: Robust Glucose and 1,5-Anhydroglucitol Uptake Assays Using Novel Fluorescent Conjugates
Submitted on 14 Mar 2018
Author(s): Shunan Li,Ph.D., Santoshkumar Khatwani,Ph.D., Yuan Lin,Ph.D., Sonika Saddar,Ph.D., Grigoriy Tchaga, Ph.D., and Gordon Yan, Ph.D.
Affiliations: BioVision,Inc
Poster Views: 585
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Poster Information
Abstract: Glucose is a primary carbon source for ATP production and biosynthesis in proliferating mammalian
cells by either glycolytic or tricarboxylic acid (TCA) cycle pathways. These pathways are tightly
regulated and their disruption leads to many metabolic diseases. Cancer cells exhibit increased
glucose uptake to support their high proliferation rate. The glucose uptake has been widely studied by
a non-radioactive fluorescence assay using a fluorescently-labeled glucose derivative, 2-NBD-Glucose.
Despite its applicability, this conjugate exhibits photo bleaching and reduced fluorescence when used
at low concentrations (<400 µM). At BioVision, we have developed a novel bright green fluorescent
conjugates (GluTracker™ and AGTracker™). Like glucose and 2-NBD-Glucose, GluTracker™ and
AGTracker™ can be taken up by cells via glucose transporters. In addition, these glucose analogs
cannot be fully metabolized by cells, and thus accumulates inside the cells to generate strong
fluorescent signals. Fluorescence generated by this fluorescent glucose analogs is proportional to the
glucose uptake by the cells, and can be used to measure glucose uptake using fluorescence
microscopy and flow cytometry. GluTracker™ and AGTracker™ provide very useful tools for the study
of Glucose and Anhydroglucitol uptake.
Summary: BioVision’s GluTracker™ (for Glucose) and AGTracker™ (for 1,5-AG) Uptake Assays
provide valuable tools for drug discovery and identifying potential modulators in glucosedependent
pathways. The assay kits are commercially available at www.biovision.com Cat
# K681-50 and K684-50.
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