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ROTEA: A CLOSED AND AUTOMATED INSTRUMENT FOR EFFICIENT CELL ISOLATION, WASHING AND CONENTRATION IN CELL THERAPY WORKFLOWS
EP33185
Poster Title: ROTEA: A CLOSED AND AUTOMATED INSTRUMENT FOR EFFICIENT CELL ISOLATION, WASHING AND CONENTRATION IN CELL THERAPY WORKFLOWS
Submitted on 21 Oct 2020
Author(s): Carl Dargitz, Sarah Daoudi, Steffany Dunn, Namritha Ravinder and Xavier de Mollerat du Jeu
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This poster was presented at International Society for Cellular Therapy 2020
Poster Views: 139
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Poster Information
Abstract: Processing and manufacturing cell and gene therapy products is of vital importance to patients and has presented numerous challenges. Rotea’s closed system counterflow centrifugation technology enables gentle cell isolation, and wash and concentrate, and can be easily automated and programmed to serve many additional workflows. Together the single use kit and instrument are able to capture and sustain billions of cells in a stable, fluidized bed with flexible inputs up to 20 liters and output volumes as low as 5 mL..

We set out to evaluate Rotea’s ability to isolate PBMCs from leukopaks, and wash and concentrate T cells, two common and vital processes in cell therapy workflows. We also investigated the input and output capabilities of the instrument.

• Rotea provides highly efficient PBMC isolation across many donors with high recovery and cell quality. It can isolate PBMCs much faster than manual methods while maintaining equivalent or improved quality and cell health.
• Rotea provides gentle and efficient cell wash and concentrate with >90% recovery of T cells, and maintenance of viability with flexible input and output volume.
• Due to it’s high level of flexibility and programmability, Rotea is capable of facilitating the whole cell therapy workflow from PBMC isolation all the way to cryopreservation.
Summary: Processing and manufacturing cell and gene therapy products is of vital importance to patients and has presented numerous challenges. Rotea’s closed system counterflow centrifugation technology enables gentle cell isolation, and wash and concentrate, and can be easily automated and programmed to serve many additional workflows.Report abuse »
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