Abstract: The Baculovirus Expression Vector System (BEVS) is a versatile platform for expression of individual recombinant proteins, multimeric protein complexes, virus-like particles (VLPs), and membrane proteins. Recently, BEVS has demonstrated particular utility in the large scale production of vaccines and viral vectors. Here, we describe the ExpiSf™ Expression System, the first chemically defined insect expression system that enables high-yield production of proteins and viral particles with consistent performance using a fast, streamlined workflow. The system demonstrates scalability for production of proteins and viral vectors in stirred tank, rocking motion, and microbioreactors. We discuss optimal bioprocessing parameters and protocols to enable high quality process development for production of targets of interest. Summary: The Baculovirus Expression Vector System (BEVS) is a versatile platform for expression of individual recombinant proteins, multimeric protein complexes, virus-like particles (VLPs), and membrane proteins. Here, we describe the ExpiSf™ Expression System and its ability to enable high-yield production of proteins and viral particles with consistent performance using a fast, streamlined workflow.References: 1.Smith,RH,Levy,JR,andKotin,RM(2009).Asimplifiedbaculovirus-AAVexpressionvectorsystemcoupledwithone-stepaffinitypurificationyieldshigh-titerrAAVstocksfrominsectcells.MolTher.11:1888-1896.2
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