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Scalable protein and AAV production in the ExpiSf™ Expression System
EP32717
Poster Title: Scalable protein and AAV production in the ExpiSf™ Expression System
Submitted on 18 Jun 2020
Author(s): Sara Barnes1, Mark Bundy1, Ken Thompson1, Maya Yovcheva1, Katy Irvin1, Melissa Cross1, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1
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Poster Information
Abstract: The Baculovirus Expression Vector System (BEVS) is a versatile platform for expression of individual recombinant proteins, multimeric protein complexes, virus-like particles (VLPs), and membrane proteins. Recently, BEVS has demonstrated particular utility in the large scale production of vaccines and viral vectors. Here, we describe the ExpiSf™ Expression System, the first chemically defined insect expression system that enables high-yield production of proteins and viral particles with consistent performance using a fast, streamlined workflow. The system demonstrates scalability for production of proteins and viral vectors in stirred tank, rocking motion, and microbioreactors. We discuss optimal bioprocessing parameters and protocols to enable high quality process development for production of targets of interest. Summary: The Baculovirus Expression Vector System (BEVS) is a versatile platform for expression of individual recombinant proteins, multimeric protein complexes, virus-like particles (VLPs), and membrane proteins. Here, we describe the ExpiSf™ Expression System and its ability to enable high-yield production of proteins and viral particles with consistent performance using a fast, streamlined workflow.References: 1.Smith,RH,Levy,JR,andKotin,RM(2009).Asimplifiedbaculovirus-AAVexpressionvectorsystemcoupledwithone-stepaffinitypurificationyieldshigh-titerrAAVstocksfrominsectcells.MolTher.11:1888-1896.2Report abuse »
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