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EP39993
Abstract: In the present study, researchers demonstrated how a cocktail of the group 1 and 2
headless HA stem-nano-particles could help elicit within-group and broadly-reactive
neutralizing bnAbs and protective immune responses against a wide range of influenza
A viruses in mice, ferrets, and non-human primates (NHPs).
The H10 stem occupies a unique position between group 2 HA stems, i.e., H3 and H7
phylogenetically, and possesses slightly higher sequence and solvent-exposed residue
identities than H3 and H7. So the researchers hypothesized that the H10-based group 2
stem immunogen(s) might have a better antigenic surface to elicit broadly cross-reactive
antibody responses against multiple influenza viruses.
The researchers used biolayer interferometry (BLI) to measure the binding kinetics of
the antigen-binding fragment of an antibody (Fab) of MEDI8852, CT149, and CR8020 to
H10ssF. They measured calcium (Ca2+) flux by flow cytometry (FC) using recombinant
Ramos B cells to evaluate the ability of H10ssF to activate B cells expressing BCRs of the
unmutated common ancestors (UCA) of human bnAbs. The team immunized mice with
H3ssF, H7ssF, or H10ssF using a sigma adjuvant system [SAS]). Likewise, they immunized
groups of mice, ferrets, and cynomolgus macaques with a cocktail of H1ssF and H10ssF
(termed herein H1+10ssF) with a squalene oil-in-water emulsion adjuvant (AddaVax).Summary: From October 2004 to February 2005, approximately 3,700 test kits of the 1957 H2N2
virus were accidentally spread around the world from the College of American
Pathologists (CAP). CAP assists laboratories in accuracy by providing unidentified
samples of viruses; private contractor Meridian Bioscience in Cincinnati, U.S., chose the
1957 strain instead of one of the less deadly avian influenza virus subtypes.[12] The 1957
H2N2 virus is considered deadly and the U.S. government References: 1. 28, 2010.^ "Encarta on influenza". Archived from the original on 2009-10-29.
Retrieved 2008-08-20.^ Anthony King: What four coronaviruses from history can tell us
about covid-19, on: New Scientist, 29 April 2020^ Jump up to:a b Green, Jeffrey (2006).
2.The Bird Flu Pandemic. St. Martin's Griffin. ISBN 0312360568.^ Belshe, Robert B.
(2005-11-24). "The Origins of Pandemic Influenza — Lessons from the 1918 Virus". New
England Journal of Medicine. 353 (21):
2209–2211. doi:10.1056/NEJMp058281. ISSN 0028-4793. PMID 16306515.
headless HA stem-nano-particles could help elicit within-group and broadly-reactive
neutralizing bnAbs and protective immune responses against a wide range of influenza
A viruses in mice, ferrets, and non-human primates (NHPs).
The H10 stem occupies a unique position between group 2 HA stems, i.e., H3 and H7
phylogenetically, and possesses slightly higher sequence and solvent-exposed residue
identities than H3 and H7. So the researchers hypothesized that the H10-based group 2
stem immunogen(s) might have a better antigenic surface to elicit broadly cross-reactive
antibody responses against multiple influenza viruses.
The researchers used biolayer interferometry (BLI) to measure the binding kinetics of
the antigen-binding fragment of an antibody (Fab) of MEDI8852, CT149, and CR8020 to
H10ssF. They measured calcium (Ca2+) flux by flow cytometry (FC) using recombinant
Ramos B cells to evaluate the ability of H10ssF to activate B cells expressing BCRs of the
unmutated common ancestors (UCA) of human bnAbs. The team immunized mice with
H3ssF, H7ssF, or H10ssF using a sigma adjuvant system [SAS]). Likewise, they immunized
groups of mice, ferrets, and cynomolgus macaques with a cocktail of H1ssF and H10ssF
(termed herein H1+10ssF) with a squalene oil-in-water emulsion adjuvant (AddaVax).Summary: From October 2004 to February 2005, approximately 3,700 test kits of the 1957 H2N2
virus were accidentally spread around the world from the College of American
Pathologists (CAP). CAP assists laboratories in accuracy by providing unidentified
samples of viruses; private contractor Meridian Bioscience in Cincinnati, U.S., chose the
1957 strain instead of one of the less deadly avian influenza virus subtypes.[12] The 1957
H2N2 virus is considered deadly and the U.S. government References: 1. 28, 2010.^ "Encarta on influenza". Archived from the original on 2009-10-29.
Retrieved 2008-08-20.^ Anthony King: What four coronaviruses from history can tell us
about covid-19, on: New Scientist, 29 April 2020^ Jump up to:a b Green, Jeffrey (2006).
2.The Bird Flu Pandemic. St. Martin's Griffin. ISBN 0312360568.^ Belshe, Robert B.
(2005-11-24). "The Origins of Pandemic Influenza — Lessons from the 1918 Virus". New
England Journal of Medicine. 353 (21):
2209–2211. doi:10.1056/NEJMp058281. ISSN 0028-4793. PMID 16306515.
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