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Screening of ALDH2 Interacting Proteins by Co-Immunoprecipitation and Mass Spectrometry
EP26863
Poster Title: Screening of ALDH2 Interacting Proteins by Co-Immunoprecipitation and Mass Spectrometry
Submitted on 08 Feb 2018
Author(s): Irene John
Affiliations: University of South Florida
This poster was presented at USF Health Research Day 2018
Poster Views: 504
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Poster Information
Abstract: Mitochondrial aldehyde dehydrogenase 2 (ALDH2) has been shown to protect against oxidative stress in multiple organs. ALDH2 has a role in preventing oxidative stress-induced 4-HNE adduction in multiple organs. As a result, ALDH2 may have an unidentified protein interaction network, which would help elucidate its importance in various cellular functions. Studies show that ALDH2 aids in protecting the lungs against oxidative stress. We are studying ALDH2 in the lung because it has been demonstrated to be critical to the integrity of lung cells and an important shield from damage occurring under oxidative stress (Chen et. al).
Since information regarding the ALDH2 interactome is limited, exploring cells which assume different cell profiles, type II AEC and club cell, may give us new insight of ALDH2’s counteractive role against oxidative stress
This results show that ALDH2 may have an unidentified protein interaction network, which would help elucidate its importance in various cellular functions. The combination of immunoprecipitation and mass spectrometry will give us insights that could not be easily attained otherwise
Summary: Recent studies have revealed that mitochondrial aldehyde dehydrogenase 2 (ALDH2) plays an important role in protecting against oxidative stress in multiple organs, including the lung. Protein-protein interaction has long been known to affect critical cellular functions. Therefore, exploring unidentified binding partners of ALDH2 could improve our understanding of how ALDH2 counteracts oxidative stress. The objective is to screen and identify and binding partners of ALDH2. References: Chen, C.-H., et al. “Targeting Aldehyde Dehydrogenase 2: New Therapeutic Opportunities.”Physiological Reviews, vol. 94, no. 1, Jan. 2014, pp. 1–34., doi:10.1152/physrev.00017.2013Report abuse »
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