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Sequence Curation and Glycoform Analysis of SARS-CoV-2 RBD Domains Produced in Mammalian Cell Lines
EP38452
Poster Title: Sequence Curation and Glycoform Analysis of SARS-CoV-2 RBD Domains Produced in Mammalian Cell Lines
Submitted on 09 Mar 2022
Author(s): 1Christoph Gstöttner, 1Tao Zhang, 2Anja Resemann, 1Sophia Ruben, 2Stuart Pengelley, 2Arndt Asperger, 2Detlev Suckau, 3Tim Welsink, 1Manfred Wuhrer, 1Elena Domínguez-Vega
Affiliations: 1Center for Proteomics and Metabolomics, LUMC, Leiden, NL 2Bruker Daltonics, Bremen, Germany 3InVivo BioTech Services, Hennigsdorf, Germany
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Poster Information
Abstract: The SARS-CoV-2 spike (S) protein’s receptor binding domain (RBD) mediates the interaction with the ACE2 receptor on host cells and is target to immune response and diagnostic tools. We characterized recombinant RBDs on the levels of released glycans, glycopeptides, intact mass analysis with glycan-enzymatic dissection and Top-Down Sequencing for comprehensive annotation of RBD proteoforms (1). This work not only offers insights into RBD structural and functional features but also provides a workflow for characterization of new RBDs and batch-to-batch comparison.Summary: This work not only offers insights into RBD structural and functional features but also provides a workflow for characterization of new RBDs and batch-to-batch comparison.References: 1. C Gstöttner, T Zhang, A Resemann, S Ruben, S Pengelley, D Suckau, T Welsink, M Wuhrer, E Domínguez-Vega. Anal. Chem. 2021, 93, 17, 6839–6847Report abuse »
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