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Sequence-Specific Ni(II)-Dependent Peptide Bond Hydrolysis for Protein Engineering. Combinatorial Library Determination of Optimal Sequences
EP20295
Poster Title: Sequence-Specific Ni(II)-Dependent Peptide Bond Hydrolysis for Protein Engineering. Combinatorial Library Determination of Optimal Sequences
Submitted on 19 Dec 2013
Author(s): Artur Krezel, Edyta Kopera, Anna Maria Protas, Jaroslaw Poznanski, Aleksandra Wyslouch-Cieszynska and Wojciech Bal
Affiliations: Institude of Biochemistry and Biophysics, Polish Academy of Sciences
Poster Views: 1,040
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Abstract: The sequence-specific cleavage of the peptide bond is a crucial procedure in protein engineering and purification. The extreme stability of this bond, with half-life for spontaneous hydrolysis estimated as 350-600 years at neutral pH and room temperature, limits the range of appropriate cleavage reagents.
Summary: The sequence-specific cleavage of the peptide bond is a crucial procedure in protein engineering and purification. The extreme stability of this bond, with half-life for spontaneous hydrolysis estimated as 350-600 years at neutral pH and room temperature, limits the range of appropriate cleavage reagents.
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