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Single-use technology in the production of DNA-free Taq DNA polymerase
EP29306
Poster Title: Single-use technology in the production of DNA-free Taq DNA polymerase
Submitted on 16 Oct 2018
Author(s): Kęstutis Bargaila, Vytautas, Budrys, Andrius Krasauskas, Dovilė Lisauskienė, Milda Romeikaitė, Sonata J.-Urbanavičienė, and Juozas Šiurkus
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Abstract: The contaminating DNA can originate from the PCR reagent manufacturing environment, raw materials, human operators, or host cells expressing recombinant proteins. The main source of DNA contamination is the enzyme used for PCR, i.e.,Taq DNA polymerase. Reported DNA removal methods vary in efficiency and may decrease the sensitivity of the assay. We introduce InvitrogenTM PlatinumTM Taq DNA Polymerase, DNA-free – a PCR enzyme with the lowest DNA contamination levels as compared to competing “DNA-free” enzymes. This was achieved through manufacturing innovation, where the enzyme is produced using a closed single-use system (SUS) to minimize the risk of DNA contamination inherent to the conventional manufacturing process.Summary: Polymerase chain reaction (PCR) technology, used for PCR-based assays, is fast, sensitive, and specific; however, uncontrolled DNA contamination in assays could compromise sensitivity and specificity.References: We would like to thank Eglė Merkienė for her support in
development of Platinum Taq DNA polymerase, DNA-free.
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