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siRNA Design Including Secondary Structure Target Site Prediction
EP20357
Poster Title: siRNA Design Including Secondary Structure Target Site Prediction
Submitted on 19 Dec 2013
Author(s): Gerrit Schramm
Affiliations: MWG Biotech
Poster Views: 870
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Poster Information
Abstract: When performing RNAi experiments using siRNAduplexes, the first critical challenge is the design of efficient siRNA. Recent studies concerning the importance of mRNA accessibility in relation to the efficiency of siRNAsilencing has led MWG Biotech to create an online design tool allowing researchers to view the mRNA target site.

Preliminary data underlines the correlation between the siRNA knockdown efficiency and the local free energy of the mRNA target site. There is a strong evidence that considering the mRNA secondary structure within the siRNA design algorithm will lead to more efficient siRNAs.
Summary: Preliminary data underlines the correlation between the siRNA knockdown efficiency and the local free energy of the mRNA target site. There is a strong evidence that considering the mRNA secondary structure within the siRNA design algorithm will lead to more efficient siRNAs.Report abuse »
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