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Specificity of highly potent miRNA inhibitors
EP22801
Poster Title: Specificity of highly potent miRNA inhibitors
Submitted on 10 Mar 2015
Author(s): Barbara Robertson, Andrew Dalby, Yuriy Fedorov, Jon Karpilow, Anastasia Khvorova1, Devin Leake, Annaleen Vermeulen
Affiliations: GE Healthcare Dharmacon, Inc.
Poster Views: 1,446
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Abstract: MicroRNAs (miRNAs) have been shown to regulate gene expression through both translational attenuation and cleavage of messenger RNAs. Though these small non-coding RNAs are predicted to play significant roles in development, differentiation, and disease etiology, validation of miRNA targets remains difficult. Highly potent miRNA inhibitors represent invaluable tools for elucidating the roles of miRNAs. However, it is important to be aware that such potent inhibitors may also affect function of other miRNAs, especially miRNA family members. To understand the potential cross-reactivity of miRNA inhibitors, various miRNA inhibitor designs were systematically tested. We demonstrate that mismatches both within and outside the seed region of the miRNA interfere with inhibition. Our findings indicate that certain features important for natural miRNA target recognition also appear to be important for inhibitor specificity. Furthermore, results from specificity experiments in a reporter assay are consistent with results obtained in an endogenous system. Understanding the specificity of inhibitors allows for better interpretation of inhibitor activity in endogenous systems.Summary: miRNA inhibitors are invaluable tools for elucidating the roles of miRNAs. However, potent inhibitors may also affect other miRNAs. To understand the potential cross-reactivity of miRNA inhibitors, various miRNA inhibitor designs were systematically tested. We demonstrate that mismatches both within and outside the seed region of the miRNA interfere with inhibition. Our findings indicate that features important for natural miRNA target recognition are also important for inhibitor specificity.References: Report abuse »
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