We've updated our Privacy Policy to make it clearer how we use your personal data.
We use cookies to provide you with a better experience, read our Cookie Policy
EP20336
Abstract: Surface Plasmon Resonance imaging (SPR imaging) is an optical technique that, in combination with microarrays, can be used to study the kinetics of multiple biomolecular interactions. The main advantage is the ability to measure in a label-free and real-time manner.
In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
By using fluorescently labelled antibodies, the binding to the immobilized proteins monitored with SPR imaging, could be confirmed on the very same sensor with fluorescence microscopy.
Summary: In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
By using fluorescently labelled antibodies, the binding to the immobilized proteins monitored with SPR imaging, could be confirmed on the very same sensor with fluorescence microscopy.
Summary: In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Related Posters
Targeted and Discovery Proteomics
Creative Proteomics
Characteristics of ITRAQ, TMT, and SILAC
Creative Proteomics
Three Methods for Protein Sequencing
Creative Proteomics
Abstract 23: Precision medicine approaches to Fanconi anemia oral cancer personalized prevention and treatment
Daniel K. Swenson; Beverly R. Wuertz; Mustafa M. Ali; Gretchen M. Unger; Frank G. Ondrey
Method of Host Cell Protein Analysis
Creative Proteomics
