« Back
Sub-regional differences in metabolic activity of human amniotic membrane - a rich source of stem cells
Sub-regional differences in metabolic activity of human amniotic membrane - a rich source of stem cells
Submitted on 06 Jan 2018

Asmita Banerjee (1), Adelheid Weidinger (1), Andrea Lindenmair (1), Simone Hennerbichler (2), Heinz Redl (1), Andrey Kozlov (1), Susanne Wolbank (1)
1 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; 2 Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria
This poster was presented at PACT symposium 2015, Vienna, Austria
Poster Views: 210
View poster »
Poster Abstract
The human amniotic membrane (hAM), the innermost fetal membrane, contains cells with stem cell characteristics with low immunogenicity, making it a suitable material for tissue engineering. For clinical application, profound knowledge of properties, differentiation capacity and quality of the applied material is a prerequisite. Previously, we have shown that hAM can be differentiated towards osteogenic, chondrogenic and Schwann cell-like lineages. Differentiation is a highly energy-consuming process. However, hAM can be partitioned in placental and non-placental regions, giving rise to the question, whether these sub-regions show differences in morphology and energy metabolism. Mitochondrial respiration, monitored by high resolution respirometry, Oroboros Instruments, was 4-fold higher in the placental region compared to the non-placental region. Interestingly, respiratory control ratio showed no differences, reflecting similar quality of mitochondria in both regions, suggesting higher numbers of mitochondria in the placental region. Thus, the placental region seems to be more capable in terms of energy production, but also more oxygen-dependent. In histological sections of hAM, stained with haematoxylin/eosin, epithelial cells of the placental region appeared cylindrical with decentralized nuclei, whereas epithelial cells of the non-placental region were flattened and more homogenous. Thus, the placental and non-placental regions show distinct differences in morphology and mitochondrial activity. Since mitochondrial activity is closely related to cell type and function, the question is whether regional differences reflect different cellular functions. If so, may this also play a role in functionality, differential potential and cell fate, and may hence impact therapeutic properties in clinical applications.Report abuse »
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters

Novel Method for the Detection of Glucose Uptake: Direct Measurement of Glucose Levels in Cultured Cells
Kevin Yan1,2 , Shunan Li1 , Yuan Lin1 , Sonika Saddar1 , Grigoriy Tchaga1 , Gordon Yan1

Casein Kinase Delta 1 Inhibitor Recovers Cognitive Deficits in APPPS1 Model of Alzheimer's Disease
A. Portugues, M.S., S. Nagaraj, A.Yunus, B.S., J.J. Gamsby, Ph.D., D. Gulick, Ph.D.

The mutated cytoplasmic form of nucleophosmin expressed by the cell line OCI-AML3 is phosphorylated on Thr199: implications for the pathogenesis of primary acute myeloid leukaemia (AML)
safaa A.A. al.

Cardiovascular complications and mortality determinants in near drowning victims: a 5 year retrospective analysis
Hesham R. Omar, MD, Mehdi Miraeidi, MD, Gerardo Bosco, MD, Kevin Morgan, MS, Prachiti Dalvi, Engy Helal, MD, Devanand Mangar, MD, Enrico M Camporesi, MD

LISPRO, an ionic co-crystal of lithium, mitigates Alzheimer-like pathological changes and associated cognitive-and neuropsychiatric behavioral deficits in transgenic Alzheimer’s mice
Ahsan Habib, Darrell Sawmiller, Selina koilraj, Sadia Afrin Munna, David Rongo, Jun Tian, Huayan Hou, Jin Zeng, Brian Giunta, Adam Smith, Takashi Mori, Glenn Currier, Douglas R Shytle, and Jun Tan