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SuperScript® IV Reverse Transcriptase: A New Reverse Transcriptase for RNA Analysis
EP29304
Poster Title: SuperScript® IV Reverse Transcriptase: A New Reverse Transcriptase for RNA Analysis
Submitted on 16 Oct 2018
Author(s): Bianca J Lam1 , Kevin Zhang1 , Joanna Guo1 , Lushen Li 1 , Kimberly Wong Garcia 1 , Asta Jokubauskaite 2 , Milda Kaniusaite2 , Tomas Radzvilavicius2 , and Arunas Lagunavicius 2
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Poster Views: 647
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Poster Information
Abstract: Survey and interview studies conducted over a three year
period revealed that researchers are not satisfied with their
current reverse transcriptase and are performing reactions
with increasingly difficult samples, such as poorly purified
RNA and unpurified RNA (direct RT) that both contain
inhibitors. To meet this performance gap, the Thermo Fisher
Life Sciences Solutions group produced a new reverse
transcriptase, SuperScript® IV, and experiments we
performed show that it is the most robust reverse
transcriptase compared to other enzymes. SuperScript® IV
characterization was performed in the context of “real
world” situations where users do not have perfect RNA
samples. In the presence of a variety of inhibitors, we
demonstrate that SuperScript® IV possesses superior
performance in a variety of inhibitors, such as alcohols,
salts, detergents, phenol, heparin, hematin, bile salts, and
formalin typically found in sample preparation reagents, cell
lines, blood, feces, and FFPE samples. This enzyme can
even detect RNA targets in unpurified RNA samples
(directly lysed cells) and whole blood without sacrificing
sensitivity and yield. The introduction of SuperScript® IV
enables researchers to obtain more consistent results
independent of sample quality and simplify and speed up
workflows by eliminating RNA purification.
Summary: Gene expression starts when RNA is transcribed from DNA.
Expression levels of different RNA targets can be used to
characterize species, tissue types, cell types, and healthy
and diseased cells. Because RNA is unstable, it is
necessary to convert these molecules into more stable
ones without loss of quantitative and coding information.
Thus, reverse transcriptases are indispensable enzymes
because they convert RNA into DNA (cDNA), a stable form
of the genetic code
References: We would like to thank Toby Jordan and Samantha Li for their
work and support in the development of SuperScript®IV
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