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EP24146
Poster Title: Symbiodinium Diversity in Porites compressa Following a Natural Bleaching Event
Submitted on 20 Jun 2016
Author(s): Martha Newell, C. Jury, I. Knapp, K. Dobson, R. McLachaln, R. Toonen, A. Grottoli, P. Marko
Affiliations: Zoology, University of Hawaii at Manoa; Hawaii Institute of Marine Biology, University of Hawaii at Manoa; Earth Sciences, The Ohio State University
This poster was presented at International Coral Reef Symposium
Poster Views: 1,730
Submitted on 20 Jun 2016
Author(s): Martha Newell, C. Jury, I. Knapp, K. Dobson, R. McLachaln, R. Toonen, A. Grottoli, P. Marko
Affiliations: Zoology, University of Hawaii at Manoa; Hawaii Institute of Marine Biology, University of Hawaii at Manoa; Earth Sciences, The Ohio State University
This poster was presented at International Coral Reef Symposium
Poster Views: 1,730
Abstract: Climate change has led to increases in coral bleaching worldwide. The question of why
corals expel their symbiotic zooxanthellae (Symbiodinium) has been studied from the
perspective of the coral host as well as the symbiont. While progress has been made
toward understanding the physiological and cellular mechanisms that cause bleaching,
Next-generation sequencing (NGS) permits further investigation of genetic explanations
for variations in bleaching response across the entire genome. This work is focused on
identifying genetic factors from the coral genome that are associated with bleaching
variation, as well as the composition of Symbiodinium communities within corals during
and after bleaching. To address the question of Symbiodinium clade composition, we
compared Symbiodinium in colonies of Porites compressa that varied in their response
during the 2014 bleaching event in Hawaii. Although other species of Porites have been
shown to host thermally-tolerant clade D zooxanthellae, previous studies have found that
P. compressa only host a single clade (C), even among colonies in habitats that have a
history of high temperatures. For this work, we genetically analyzed colonies of P. compressa
sampled from October 2014 through November 2015 from four sites in the Main
Hawaiian Islands. Our approach is to use restriction site-associated DNA sequencing
(RADseq) to analyze genomes of the coral holobiont and identify symbiont clades, and
we will discuss progress in using NGS to characterize symbiont diversity among colonies
with different bleaching phenotypes.Summary: This is an examination of the algal symbionts (Symbiodinium) in Porites compressa following the 2014 bleaching event in the Main Hawaiian Islands. We specifically were interested in which Symbiodinium clades were present in the coral tissue after bleaching. The clades were identified through single gene alignment and de novo assemblies of contigs to BLAST against the NCBI databaseReferences: 1.Rowan, R., Knowlton, N., Baker, A., Jara, J. 1997. Nature 338:265-269
2.Stat, M., Pochon, X et al 2013. Ecology and Evolution 3(5);1317-1329
3. Pochon, X., Putnam, H., Burki, F., Gates, R. 2012. PlosONE 7(1):e29816
corals expel their symbiotic zooxanthellae (Symbiodinium) has been studied from the
perspective of the coral host as well as the symbiont. While progress has been made
toward understanding the physiological and cellular mechanisms that cause bleaching,
Next-generation sequencing (NGS) permits further investigation of genetic explanations
for variations in bleaching response across the entire genome. This work is focused on
identifying genetic factors from the coral genome that are associated with bleaching
variation, as well as the composition of Symbiodinium communities within corals during
and after bleaching. To address the question of Symbiodinium clade composition, we
compared Symbiodinium in colonies of Porites compressa that varied in their response
during the 2014 bleaching event in Hawaii. Although other species of Porites have been
shown to host thermally-tolerant clade D zooxanthellae, previous studies have found that
P. compressa only host a single clade (C), even among colonies in habitats that have a
history of high temperatures. For this work, we genetically analyzed colonies of P. compressa
sampled from October 2014 through November 2015 from four sites in the Main
Hawaiian Islands. Our approach is to use restriction site-associated DNA sequencing
(RADseq) to analyze genomes of the coral holobiont and identify symbiont clades, and
we will discuss progress in using NGS to characterize symbiont diversity among colonies
with different bleaching phenotypes.Summary: This is an examination of the algal symbionts (Symbiodinium) in Porites compressa following the 2014 bleaching event in the Main Hawaiian Islands. We specifically were interested in which Symbiodinium clades were present in the coral tissue after bleaching. The clades were identified through single gene alignment and de novo assemblies of contigs to BLAST against the NCBI databaseReferences: 1.Rowan, R., Knowlton, N., Baker, A., Jara, J. 1997. Nature 338:265-269
2.Stat, M., Pochon, X et al 2013. Ecology and Evolution 3(5);1317-1329
3. Pochon, X., Putnam, H., Burki, F., Gates, R. 2012. PlosONE 7(1):e29816
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