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Targeting Induced Oral Squamous Cell Carcinoma Using Panel of Chemotherapeutics Loaded on Gold Nanoparticles (Experimental Study)
Poster Title: Targeting Induced Oral Squamous Cell Carcinoma Using Panel of Chemotherapeutics Loaded on Gold Nanoparticles (Experimental Study)
Submitted on 22 Feb 2019
Author(s): Marwa M. Essawy1,2, Sagy L. Assar1, Hend M. Abdel Hamid1, Sahar M. El-Sheikh1, Fatma H. El-Didi1, Zeinab E. Darwish1, Hanaa S. Raslan1, Ghada M. Mourad2,3, Marwa M. Afifi1.
Affiliations: 1. Oral Pathology Department, Faculty of Dentistry, Alexandria University, Egypt. 2. Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Egypt. 3. Histology and Cell Biology Department, Faculty of Medicine, Alexandria University, Egypt.
This poster was presented at Oral & Maxillofacial Pathology Scientific Day, Cairo University, Egypt
Poster Views: 1,574
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Poster Information
Abstract: INTRODUCTION: Oral squamous cell carcinoma (OSCC) is the sixth common cancer. The use of nanoparticles in cancer therapy as nanoparticle-based drug delivery system is attractive. Gold nanoparticles (AuNPs) are an excellent candidate for drug delivery vehicles due to their unique physical and chemical properties.
OBJECTIVES: The study aimed to evaluate and compare actively targeting cancer cells with 4 different chemotherapeutic drugs Doxorubicin (DOX), 5-flourouracil (5FU), Placlitaxel (PTX) and Camptothecin (CPT) loaded on gold nanoparticles. The treatment was evaluated by the use of proliferative immunhistochemical marker.
METHODOLOGY: Oral SCC was chemically induced in 100 Syrian golden hamsters. After cancer development they were divided into 8 main groups 4 groups received functionalized AuNPs with the 4 drugs (DOX-AuNPs, 5FU-AuNPs, PTX-AuNPs, CPT-AuNPs) separately. Another 4 groups treated with the free form of the 4 drugs and 2 groups acted as a control receiving saline and free AuNPs without any loaded drug. All the groups were treated using intraperitoneal (IP) route of drug administration. The proposed treatment was evaluated clinically in terms of change in tumor volume and survival rate, whereas, the histological evaluation was in term of calculating the apoptotic index (AI). The proliferative activity was evaluated immunohistochemical by Proliferation cell nuclear antigen (PCNA) immunstain. In all statistical results, a p < 0.05 was considered significant.
RESULTS: Hamsters treated with DOX-AuNPs and 5FU-AuNPs showed the highest significant decrease in mean tumor volume and the longest survival rate. They were followed by PTX-AuNPs, then finally CPT-AuNPs. The AI also reported the highest significant value in DOX-AuNPs group and the least value was recorded by CPT-AuNPs group. The PCNA immune-staining revealed significant decrease in the values of mean area percent and mean optical density in all groups with the lowest value was seen in hamsters receiving DOX-AuNPs.
CONCLUSION: Gold NPs can be used efficiently as a drug delivery system in the treatment of OSCC. Doxorubicin as well as 5FU can be used as excellent candidates targeting OSCC.
Summary: Using gold nanoparticles as drug delivery system in the treatment of oral squamous cell carcinoma. loading the nanoparticle with panel of chemotherapeutic drugs and studying their short-term anti-cancer efficiency. References: 1.Kitakawa D, Cabral LA, Marques ME, Salvadori DM, Ribeiro DA. J Exp Anim Sci 2006;43:219–227.
2.Ghosh D, Sarkar D, Girigoswami A, Chattopadhyay N. J Nanosci Nanotechnol 2010;10:1–6.
3.Afifi MM, Austin LA, Mackey MA. El-Sayed MA. Bioconjug Chem 2014;25:207–215.
4.Keith WN, Mee PJ, Brown R. Cancer Res 1990;50:6841–7.
5.Afifi MM, El Sheikh SM, Abdelsalam MM, Ramadan H, Omar TA, El Tantawi M, et al. Oral Surg Oral Med Oral Pathol Oral Radiol 2013;115:743–751.
6.Myoung H, Kim MJ, Lee JH, Ok YJ, Paeng JY, Yun PY. Int J Oral Maxillofac Surg 2006;35:1005–1010.
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