Posters
« Back
Testing a Novel Real Time Cell Viability Assay
EP23504
Poster Title: Testing a Novel Real Time Cell Viability Assay
Submitted on 09 Oct 2015
Author(s): Amy Landreman, Sarah Duellman, Wenhui Zhou, Jolanta Vidugiriene, Brad Hook
Affiliations: Promega Corporation
Poster Views: 2,021
View poster »


Poster Information
Abstract: Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light. The real time viability assay is non-toxic to cells, so viable cells remain in the sample well following measurement of the live cell signal. In addition to providing real time kinetic measurements that are valuable for assay development and characterization activities, multiplexing with other assays (e.g. dead cell staining, apoptosis, oxidative stress markers, reporter gene assays or RNA extraction) provides a time saving approach and statistical advantage inherent in taking measurements from the same sample of cells.Summary: Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light. References: Amy Landreman, Sarah Duellman, Wenhui Zhou, Jolanta Vidugiriene, Brad Hook
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Bleeding Pseudoaneurysm in uncommon locations: interesting cases
Dr Joel James (SHO), Dr Chandni Patel (SpR) & Dr Shirish Prabhudesai (Consultant)

Streamlining Biologics Development with the Expi Expression Systems
Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Kenneth Thompson, Maya Yovcheva, Sara Barnes, Mark Bundy, Melissa Cross, Katy Irvin, Joaquín Canay, Mary Reynolds, Natasha Lucki, Henry Chiou, Andrew Campbell, Jonathan Zmuda

ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda

Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A

"Juggling Fire in the Jungle" My journey of thirty years living in a sustainable community experiment
Graham Ellis