Posters
« Back
Testing a Novel Real Time Cell Viability Assay
EP23504
Poster Title: Testing a Novel Real Time Cell Viability Assay
Submitted on 09 Oct 2015
Author(s): Amy Landreman, Sarah Duellman, Wenhui Zhou, Jolanta Vidugiriene, Brad Hook
Affiliations: Promega Corporation
Poster Views: 1,808
View poster »


Poster Information
Abstract: Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light. The real time viability assay is non-toxic to cells, so viable cells remain in the sample well following measurement of the live cell signal. In addition to providing real time kinetic measurements that are valuable for assay development and characterization activities, multiplexing with other assays (e.g. dead cell staining, apoptosis, oxidative stress markers, reporter gene assays or RNA extraction) provides a time saving approach and statistical advantage inherent in taking measurements from the same sample of cells.Summary: Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light. References: Amy Landreman, Sarah Duellman, Wenhui Zhou, Jolanta Vidugiriene, Brad Hook
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Functional Assay of Neural Activity with Cell-Based Neural Culture Models and Microelectrode Array Technology for Proconvulsant Risk Assessment in the Neutox Pilot Study
Millard, D.C.; Hayes, H.B.; Nicolini, A.M.; Arrowood, C.A.; Clements, M;

Microstructure Analysis of Cr-Mo-V-W High-Entropy Alloys
Daiki Ikeuchi

Assessment of Radiation Protection Awareness and Knowledge about Common Radiological Examination Doses among Healthcare Workers in Riyadh, Saudi Arabia
Alyousef K, Assiri A, Almutairi S, Aldalham T, Felimban G

The Good, the Bad and the Ugly: Selective single cell isolation
Sandra Lubos1,2, Nils K├Ârber1, Heide Marie Resch1, Iris Augustin2, Stefan Niehren1

Osteolytic jaw lesions
Dr. Amal Hassan Zakarya, Sarah Ahmed Mohamed, Noura Mohamed Kamal, Heba Ahmed Saleh, Ali Abd Elwahed