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The effect of Wharton
EP29766
Poster Title: The effect of Wharton's jelly mesenchymal stem cells conditioned medium on Squamous cell carcinoma cell line.
Submitted on 16 Feb 2019
Author(s): Moataz El-Kholy (1), Mohamed S. Ayoub (2) and Mohamed H. El-Mallahy (2)
Affiliations: (1) Lecturer of Oral Pathology, MSA University, (2) Professor of Oral Pathology, Ain Shams University
This poster was presented at Faculty of Oral Medicine, Cairo University
Poster Views: 139
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Poster Information
Abstract: Title: This experiment explores the effect of effect of Wharton’s jelly mesenchymal stem cells (WJMSCs)-conditioned medium on the HEP-2 cell line.
Background: Wharton’s jelly mesenchymal stem cells may be more useful for cancer therapy than other adult stem cells since they are easily prepared in relatively large quantities from the umbilical cord after delivery. This study aims to determine the anticancer effect of WJMSC-conditioned medium on a HEP-2 cell line after 24, 48, and 72 hours using flow cytometry and real-time polymerase chain reaction.
Methods and findings: A HEP-2 cell line and WJMSCs were propagated. The cultured medium of WJMSCs was collected and used to substitute the cultured medium of the HEP-2 cell line. The HEP-2 cells were assessed after 24, 48, and 72 hours. We found that WJMSC-conditioned medium caused apoptosis in the HEP-2 cell line. The percentage of apoptosis was directly proportional to time. Expression of p53 was increased after 24 and 48 hours, but after 72 hours its expression decreased. The expression of Bcl-2 also decreased over time.
Conclusions: It could be concluded that WJMSC-conditioned medium could be used as an apoptotic agent against malignant cells.
Summary: This Study aimed to evaluate the effect of Wharton's jelly mesenchymal stem cell conditioned medium on squamous cell carcinoma cell line.References: 1- Batsakis J (1979) Tumors of the head and neck. Baltmore: Williams
and Wilkins.
2- Dimery I, Hong W(1993) Overview of combined modality therapies
for head and neck cancer. J Natl Cancer Inst 85: 95-111.
3- Yuan Y, Liao Y, Hsueh C, Mirshahidi H (2011) Novel targeted therapeutcs: Inhibitor of MDM2, ALK and PARP. J Haemtol Oncol 4: 16.
4- Bhardwaj A, Singh S, Singh A (2010) MicroRNA-based cancer
therapeutcs: Big hope from small RNAs. Mol Cell Pharmacol 2: 213-219.
5- Troyer D, Weiss M (2008) Wharton’s jelly-derived cells are a primitve
stromal cell populaton. Stem Cells 26: 591-59.
6- Ganta C, Chiyo D, Ayuzawa R, Rachakatla R, Pyle M, et al. (2009)
Rat umbilical cord stem cells completely abolish rat mammary
carcinomas with no evidence of metastasis or recurrence 100 days
post-tumor cell inoculaton. Cancer Res 69: 1815-1820.
7- Gauthaman K, Yee F, Cheyyatraivendran S, Biswas A, Choolani
M, et al. (2012) Human umbilical cord Wharton
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