Posters
« Back
The global significance of light-driven rhodopsin proton pumps in eukaryotic marine phytoplankton
EP21681
Poster Title: The global significance of light-driven rhodopsin proton pumps in eukaryotic marine phytoplankton
Submitted on 17 Mar 2014
Author(s): Jan Strauss, Shiqiang Gao, Joe Morrissey, Chris Bowler, Georg Nagel, Thomas Mock
Affiliations: School of Environmental Sciences, University of East Anglia, Norwich, UK; Julius-von-Sachs-Institute of Biosciences, University of Würzburg, Germany; Department of Biology, Ecole Normale Supérieure, Paris, France
This poster was presented at 2014 Ocean Sciences Meeting
Poster Views: 2,307
View poster »


Poster Information
Abstract: Sunlight is the ultimate source of energy for almost all organisms. The conversion of light energy into chemical energy (phototrophy) is powered by two mechanistically distinct processes, employing chlorophyll-containing photochemical reaction centres and membrane-embedded rhodopsin photoreceptors. Rhodopsins powering phototrophy in prokaryotes were recently also discovered in eukaryotic marine phytoplankton, including the diatom Fragilariopsis cylindrus. Homologous sequences were found abundant in a global metatranscriptome survey of marine eukaryotic phytoplankton, indicating their ecological importance. Functional analysis using two-electron voltage clamp in combination with the Xenopus laevis oocyte expression system provided direct experimental evidence for light-dependent proton pumping. Additionally, absolute gene expression analysis using RT-qPCR showed highest transcript abundances in iron-limited cultures and the analysis of rhodopsin transcript abundances in eukaryotic metatranscriptomes showed significant correlations (R2=0.917) with dissolved iron concentrations in ocean surface waters. Furthermore, transgenic diatoms complemented with Fragilariopsis rhodopsin showed enhanced growth during iron limitation. Together, these data provide strong evidence for the global significance of rhodopsins from eukaryotic marine phytoplankton in iron-limited areas of the ocean by providing a trace metal-independent mechanism to photosynthesis.Summary: Rhodopsin proton pumps from eukaryotic marine phytoplankton are studied using genomics-enabled approaches that elucidate their physiological role and show their global significance in iron-limited oceans.References: Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


riboPOOL: Affordable Custom/Ribosomal RNA Depletion Against Any Species for RNA-Seq
Catherine Goh1, Andrew Walsh1, Michaela Beitzinger1, Jonas Bertram1, Kristina Döring3, Sascha Dietrich3, Iana Kim4, Stefan Kotschote2, Claus Kuhn4, Konrad Förstner3, Michael Bonin2, Stefan Hannus1, Michael Hannus1

The Good, the Bad and the Ugly: Selective single cell isolation
Sandra Lubos1,2, Nils Körber1, Heide Marie Resch1, Iris Augustin2, Stefan Niehren1

Single-cell studies: Focus on reverse transcription
Zucha Daniel, Androvic Peter, Kubista Mikael, Valihrach Lukas

Osteolytic jaw lesions
Dr. Amal Hassan Zakarya, Sarah Ahmed Mohamed, Noura Mohamed Kamal, Heba Ahmed Saleh, Ali Abd Elwahed

Potential therapeutic effects of induced pluripotent stem cells on induced salivary gland cancer in experimental rats
Yasmine Alaa El-Din1, Dina Sabry2, Amal Hassan Abdelrahman3, and Safa Fathy3