Abstract: Here scientists propose an experimental model where the replenishment of the depleted stem cell compartment of human skin may be explored using a dermal epidermal equivalent system, composed by 50 Gy irradiated extracellular collagen matrix, adipocytes derived mesenchymal stem cells (ADSCs) and normal keratinocytes.Summary: The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
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