Posters
« Back
The Preterm Infant Microbiome
EP25396
Poster Title: The Preterm Infant Microbiome
Submitted on 08 Feb 2017
Author(s): Samia Dutra, Maureen Groer, Ming Ji, Allyson Duffy, Amy D’Agata, Kathleen Armstrong, Larry Dishaw, Elizabeth Miller, Alyson Yee, Jack Gilbert
Affiliations: College of Nursing, College of Medicine, and Department of Anthropology, University of South Florida, Tampa, FL. Argonne National Laboratory, Chicago, IL.
Poster Views: 1,131
View poster »


Poster Information
Abstract: The study aims to describe and analyze the bacterial taxa of the VLBW infants gut microbiome.
Methods: We analyzed weekly stool samples from 78 VLBW infants (<1500 Gms) collected during the first 6 weeks of their NICU stay. Samples were forwarded to the care of Dr. Jack Gilbert. Stool swab samples were stored at -80oC prior to use. Microbial genomic DNA was extracted using the PowerSoil-htp 96 Well Soil DNA Isolation Kit (MoBio). The microbial content of longitudinal fecal samples during the first 6 weeks was profiled with 16SrRNA sequencing. PCR of the 16S rRNA V4 region (515F-806R) was performed and sequenced using the Illumina MiSeq platform to generate ~100,000 250 bp paired end reads per sample. Approval for this study was obtained from the University of South Florida Institutional Review Board. All participants gave informed written consent to donate their children’s stool samples prior to their participation.
Results: Our study population consist of 78 VLBW infants with a mean gestational age of 28.3 weeks (range 24 to 34.4), and a mean birth weight of 1100.9 gms (range 600-1485 gms). The gender distribution was equal. Length of stay mean was 69 days, with a range of 20 to 215 days. Thirty-eight of the infants suffered respiratory distress syndrome and the mean days on oxygen were 14.3 days (range of 0-101 days).
The microbiota were low in diversity with phyla composition of 46.7% Proteobacteria, 22.2% Firmicutes, 4.6% Actinobacteria, and 0.4% Bacteroidetes. The phylum Proteobacteria was dominated by gammaproteobacteria (46.7%) and the family Enterobacteriaceae (46.1%).
Discussion: The preterm infant microbiota described in our study are largely consistent with other findings (Karlsson, Molin, Cilio, & Ahrné, 2011; Sharon et al., 2013), confirming an association between low gestational age and the microbial profile. The premature infant microbiome differs strongly from late term infants vaginally delivered and breastfed, whose microbiome is Actinobacteria dominated (Sharon et al., 2013). This dysbiosis is linked with detrimental outcomes for preterm infants, such as necrotizing enterocolitis and sequelae on general health of the infant host (Dogra et al., 2015; Groer et al., 2014). This occurs because shorter gestational duration may be associated with gut immaturity as well as lag in microbiota progression (Subramanian et al., 2014). Proteobacteria often account for higher proportions after antibiotic administration and in patients with gastrointestinal diseases(Hollister, Gao, & Versalovic, 2014).The family Enterobacteriaceae accounts for gram-negative bacteria that includes Salmonella, Escherichia coli, Serratia, and Citrobacter.
Conclusion: The preterm infant intestinal microbial profile may be associated with higher occurrences of gastrointestinal diseases. As an important regulator of physiological and pathophysiological effects, it is relevant to develop intervention approaches to decrease dysbiosis and increase diversity. In pursuit of appropriate interventions, studies which explore microbial influence, diversity and succession are crucial.
Summary: The infant gastrointestinal microflora is likely to significantly contribute to the core community that makes up the adult ‘signature microbiome’, which may participate in physiological and pathophysiological interactions with the host. The proposed study explored the gut microbiome profile of Very Low Birth Weight (VLBW) infants by analyzing 6 weeks of preserved stool samples while they were admitted to the Neonatal Intensive Care Unit.References: Dogra, S., Sakwinska, O., Soh, S.-E., Ngom-Bru, C., Brück, W. M., Berger, B., & Chong, Y.-S. (2015). Dynamics of infant gut microbiota are influenced by delivery mode and gestational duration and are associated with subsequent adiposity. MBio, 6(1), e02419-02414.
Groer, M. W., Luciano, A. A., Dishaw, L. J., Ashmeade, T. L., Miller, E., & Gilbert, J. A. (2014). Development of the preterm infant gut microbiome: a research priority. Microbiome, 2(1), 1.
Hollister, E. B., Gao, C., & Versalovic, J. (2014). Compositional and functional features of the gastrointestinal microbiome and their effects on human health. Gastroenterology, 146(6), 1449-1458.
Karlsson, C. L., Molin, G., Cilio, C. M., & Ahrné, S. (2011). The pioneer gut microbiota in human neonates vaginally born at term—a pilot study. Pediatric research, 70(3), 282-286.
Sharon, I., Morowitz, M. J., Thomas, B. C., Costello, E. K., Relman, D. A., & Banfield, J. F. (2013). Time series community genomics analysis revea
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Resident: DKA: A Story of Access to Insulin
Erik Rodriguez, MD., Vaidehi Ambai, DO., Viktoria Nurpiesov, MD., G.E., Alan Dever, MD, PhD.

Resident: OPIOIDS: A Case Of Rhabdomyolysis Due To Alcohol And Opioid Abuse
Aileen Polanco, MD, Ibraheem Innab, MD, Paul Mathieu, MD, Samina Fakhr, MD, Victoria Nurpiesov, MD, G.E. Alan Dever, MD, PhD,

ANEURYSM ENCASED IN ANTERIOR SKULL BASE MENINGIOMA : REVIEW OF TWO CASES
Dr Raghavendra H

Resident: Neutropenic Fever with Acute Diarrheal Illness
Juilett Kostanjevec, D.O. PGY 2; Nida Jiwani, D.O. PGY 1; Daniel Frilingos, M.D.; Amimi Osayande, M.D., FAAFP.

Resident: Strickland Weight Loss Initiative
Syed Ahmed M.D. PGY3; Brandon Alyas M.D, PGY 3, Anant Patel D.O. PGY1; Pedro Ramirez M.D. PGY1