Posters
« Back
Top-Down, Middle-Down and Bottom-Up Choose the Suitable Proteomics Strategy for Your Project
EP32614
Poster Title: Top-Down, Middle-Down and Bottom-Up Choose the Suitable Proteomics Strategy for Your Project
Submitted on 22 May 2020
Author(s): Creative Proteomics
Affiliations: Creative Proteomics
Poster Views: 261
View poster »


Poster Information
Abstract: Bottom-up is a traditional method that digests large fragment mixtures of proteins/enzymes into small fragments of peptides for analysis. It is a mass spectrometry technique widely used in proteomics research.

Middle-down proteomics strategy uses different enzymes to obtain longer peptides. It can analyze and identify several simultaneous posttranslational modifications on longer peptide chains. Compared to bottom-up method, it can analyze a wider range of peptides.

Top-down proteomics strategy does not need the enzyme digestion process, but directly analyzes the complete protein. This technique can provide more accurate and rich biological information of the complete protein.
Summary: Bottom-up is a traditional method that digests large fragment mixtures of proteins/enzymes into small fragments of peptides for analysis. It is a mass spectrometry technique widely used in proteomics research.
References: https://www.creative-proteomics.com/Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons