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Transcreener™ PDE Assay: Homogenous AMP and GMP Detection
EP20436
Poster Title: Transcreener™ PDE Assay: Homogenous AMP and GMP Detection
Submitted on 19 Dec 2013
Author(s): Matt Staeben, Rebecca Schall Josvai, Thane A. Westermeyer, Tony A. Klink, Jennifer A. Fronczak, and Karen M. Kleman-Leyer
Affiliations: BellBrook Labs
Poster Views: 1,402
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Poster Information
Abstract: Phosphodiesterases (PDEs) control a myriad of cellular processes through their ability to hydrolyze the second messenger signaling molecules, cAMP and cGMP.  This superfamily, which is grouped into 11 different gene families generating 50 different forms of PDE, has been recognized as drug targets in the therapeutic treatment of congestive heart failure, thrombosis, hypertension, depression, and erectile dysfunction.

To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP.

The Transcreener PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies. The use of a far red tracer minimizes the potential for compound interference. Here, we describe the development of the Transcreener PDE Assay and its application in identifying compounds that inhibit their activity.
Summary: To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP. The Transcreener™ PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies. Report abuse »
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