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Transfection Efficiency and Cytotoxicity of Commonly Used siRNA Transfection Reagents
EP20220
Poster Title: Transfection Efficiency and Cytotoxicity of Commonly Used siRNA Transfection Reagents
Submitted on 19 Dec 2013
Author(s): Maria Majorek, Monika Lamparska-Przybysz, Piotr Guzenda and Maciej Wieczorek
Affiliations: Celon Pharma
Poster Views: 1,937
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Poster Information
Abstract: The present study was focused on the comparison of efficiency and cytotoxicity of five most popular transfection reagents: Lipofectamine 2000, Lipofectamine RNAi MAX (Invitrogen), Dharmafect (Dharmacon), HyperFect (Qiagen) and Interferin (PolyTransfection). In this study we observed that except Lipofectamine RNAi MAX, other reagents have relatively high cytotoxicity and low transfection efficiency in MCF-7 and HeLa cells.Summary: In this study we observed that except Lipofectamine RNAi MAX, other reagents have relatively high cytotoxicity and low transfection efficiency in MCF-7 and HeLa cells.Report abuse »
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