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Ultra sensitive proteome quantification on the timsTOF SCP mass spectrometer
EP38459
Poster Title: Ultra sensitive proteome quantification on the timsTOF SCP mass spectrometer
Submitted on 09 Mar 2022
Author(s): Kristina Marx ; Christoph Krisp; Renata Blatnik; Verena Tellström ; Markus Lubeck; Oliver Raether; Nagarjuna Nagaraj; Gary Kruppa
Affiliations: Bruker Daltonics GmbH & Co.KG, Bremen, Germany
Poster Views: 364
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Abstract: The timsTOF SCP is the first commercially introduced mass spectrometer dedicated for single cell proteomics. The modified front end (orthogonal ion guiding, fig. 1) and the brighter ion beam (wider glass capillary) increase the ion transfer up to five times with keeping ultra high robustness. Here, we demonstrate the performance of the instrument for low sample loads in the range of 125 pg to 25.6 ng in combination with robust low flow rate delivery from the Evosep system.Summary: Here, we demonstrate the performance of the instrument for low sample loads in the range of 125 pg to 25.6 ng in combination with robust low flow rate delivery from the Evosep system.References: (1) https://doi.org/10.1101/2020.12.22.423933
(2) https://doi.org/10.1101/2021.01.25.427969
(3) Demichev et al .; Nat Methods 2020, 17: 41 44
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