Abstract: Long-read sequencing and miniaturization of library preparations are becoming increasingly common as new next-generation sequencing workflows are developed. Traditional quality control methods do not provide the required sizing accuracy of DNA greater than 50kb or the sensitivity allowing for sample conservation during the quality control assessment steps. The Femto Pulse system by Agilent Technologies works to streamline quality control by separating genomic DNA up to 165kb in as little as 70 minutes, down from the 16+ hours required for traditional agarose PFGE. The unparalleled single cell gDNA sensitivity of the Femto Pulse allows for preparation of low input NGS libraries from cfDNA, RNA, and miniaturized traditional DNA NGS libraries. Quality control metrics such as the RNA Quality Number (RQN) and user defined Genomic Quality Number (GQN) aids in the determination of sample quality/integrity. This poster shows the unique use of the Femto Pulse System in high molecular weight gDNA separation and low input library preparation with subsequent analysis features highlighted.Summary: This poster shows the unique use of the Femto Pulse System in high molecular weight gDNA separation and low input library preparation with subsequent analysis features highlighted.
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