Posters
« Back
Using the Promega GloSensor™ cAMP technology on the FLIPR® Tetra system for live cell Gi- and Gs- coupled GPCR second messenger assays
EP20393
Poster Title: Using the Promega GloSensor™ cAMP technology on the FLIPR® Tetra system for live cell Gi- and Gs- coupled GPCR second messenger assays
Submitted on 18 Dec 2013
Author(s): J. Pschorr, S. Lydford, C. Crittenden and Y.-W. Chen
Affiliations: Molecular Devices
Poster Views: 2,058
View poster »


Poster Information
Abstract: The poster also demonstrates endogenous receptor activity in CHO-K1 and HEK-293 cell lines stably expressing the GloSensor plasmid. In addition, transfected Gs- and Gi-coupled receptor activity are measured from cell lines with stably transfected GPCR receptors and transient transfection of the GloSensor plasmid. Multiplexing Gq- and Gs-coupled GPCR assays was shown to be possible using the GloSensor cAMP assay and FLIPR® Calcium 5 Kit in the same well. Combined with the GloSensor cAMP Assay, the FLIPR® Tetra system delivers the complete solution for kinetic screening of the major classes of GPCR subtypes.Summary: Detection of Gs- and Gi-coupled GPCR second messenger signal activity has been traditionally accomplished using endpoint assays such as radioactive binding or cAMP assays that require cell lysis. This poster demonstrates the use of the modified luminescent firefly luciferase-based Promega GloSensor™ cAMP Assay on the FLIPR® Tetra system to enable detection of cAMP mediated Gs- and Gi-coupled GPCR activity in a true kinetic assay.References: Report abuse »
Creative Commons

Related Posters


A Novel Enzymatic Assay for Determination of Phosphatidylinositol in Biological Samples
Paul Templeton, Kyle C. Schmitt, Grigoriy Tchaga,and Gordon Yan

A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells
Maya Yovcheva, Sara Barnes, Kenneth Thompson, Melissa Cross, Katy Irvin, Mintu Desai, Natasha Lucki, Henry Chiou, Jonathan Zmuda

Development of an in vitro Model System for Newcastle Disease Virus Persistence in Bladder Cancer Cells
Ahmad U1,, Chan SC2, Chau DM1, Chia SL5, Abdullah S1,3, Yusoff K5 & Veerakumarasivam A1,4*

NEW INSULAR RED PROPOLIS FROM COLOMBIA: BOTANICAL ORIGIN, BIOLOGICAL AND CHEMICAL MARKERS
Salamanca Grosso, G.; Osorio Tangafarife, M.P.

The EurOPDX EDIReX project: towards a European Research Infrastructure on patient-derived cancer models
E. Vinolo 1, J.P. Morris 1, D.G. Alférez 2, J. Arribas 3,4,5, C. Bernadó 3,4,5, A. Bertotti 6, A. Bruna 7, A.T. Byrne 8, C. Caldas 7, R.B. Clarke 2, N. Conte 9, R. Corsi 10, S. Corso 6, M. Crespo 3, A. Dahmani 11, V. Dangles-Marie 11, D. Decaudin 11, Z. Dudová 12, A. Fiori 6, S. Giordano 6, M. Hauptsmann 13, M. Hidalgo 14, C. Isella 6, S. de Jong 15, J. Jonkers 13, A. Křenek 12, O. Krijgsman 13, D. Kouřil 12, J.C. Lacal 14, L. Lanfrancone 16, E. Leucci 17, G.M. Mælandsmo 18, E. Marangoni 11, J. Mason 9, M.Th. Mayrhofer 19, A. Mazzocca 6, T.S. Meehan 9, E. Montaudon 11, F. Nem